Misp RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:153498 Diez-Roux G, et al., A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol. 2011;9(1):e1000582
Assay type:	RNA in situ
MGI Accession ID:	MGI:4822797
Gene symbol:	Misp
Gene name:	mitotic spindle positioning

Probe:	IMAGE clone 875462
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing. A tyramide-biotin/streptavidin amplification step was included in the in situ hybridization procedure.

Results

euxassay_000897_06 ©

Specimen euxassay_000897_06: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: stomach	Weak	Regionally restricted	euxassay_000897_06

euxassay_000897_07 ©

Specimen euxassay_000897_07: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: stomach	Strong	Uniform	euxassay_000897_07
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_07
TS23: midgut	Strong	Uniform	euxassay_000897_07

euxassay_000897_08 ©

Specimen euxassay_000897_08: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: stomach	Weak	Regionally restricted	euxassay_000897_08
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_08
TS23: midgut	Moderate	Regionally restricted	euxassay_000897_08

euxassay_000897_09 ©

Specimen euxassay_000897_09: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: stomach	Weak	Regionally restricted	euxassay_000897_09
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_09
TS23: midgut	Moderate	Regionally restricted	euxassay_000897_09

euxassay_000897_10 ©

Specimen euxassay_000897_10: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: stomach	Moderate	Regionally restricted	euxassay_000897_10
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_10
TS23: midgut	Moderate	Regionally restricted	euxassay_000897_10

euxassay_000897_11 ©

Specimen euxassay_000897_11: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: stomach	Moderate	Regionally restricted	euxassay_000897_11
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_11
TS23: midgut	Moderate	Regionally restricted	euxassay_000897_11

euxassay_000897_12 ©

Specimen euxassay_000897_12: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_12
TS23: midgut	Moderate	Regionally restricted	euxassay_000897_12

euxassay_000897_13 ©

Specimen euxassay_000897_13: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_13
TS23: midgut	Moderate	Regionally restricted	euxassay_000897_13
TS23: foregut-midgut junction	Moderate	Regionally restricted	euxassay_000897_13

euxassay_000897_14 ©

Specimen euxassay_000897_14: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_14
TS23: midgut	Moderate	Regionally restricted	euxassay_000897_14
TS23: foregut-midgut junction	Moderate	Regionally restricted	euxassay_000897_14

euxassay_000897_15 ©

Specimen euxassay_000897_15: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_15
TS23: midgut	Moderate	Regionally restricted	euxassay_000897_15
TS23: hindgut	Moderate	Regionally restricted	euxassay_000897_15
TS23: foregut-midgut junction	Moderate	Regionally restricted	euxassay_000897_15

Specimen euxassay_000897_16: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_16
TS23: midgut	Moderate	Regionally restricted	euxassay_000897_16
TS23: hindgut	Moderate	Regionally restricted	euxassay_000897_16
TS23: foregut-midgut junction	Moderate	Regionally restricted	euxassay_000897_16

Specimen euxassay_000897_17: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_17
TS23: midgut	Moderate	Regionally restricted	euxassay_000897_17
TS23: hindgut	Moderate	Regionally restricted	euxassay_000897_17
TS23: foregut-midgut junction	Moderate	Regionally restricted	euxassay_000897_17

Specimen euxassay_000897_18: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: midgut loop	Strong	Regionally restricted	euxassay_000897_18
TS23: foregut-midgut junction	Moderate	Regionally restricted	euxassay_000897_18

Specimen euxassay_000897_19: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: foregut-midgut junction	Moderate	Regionally restricted	euxassay_000897_19

Specimen euxassay_000897_20: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: foregut-midgut junction	Moderate	Regionally restricted	euxassay_000897_20

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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