Pclaf RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:153498 Diez-Roux G, et al., A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol. 2011;9(1):e1000582
Assay type:	RNA in situ
MGI Accession ID:	MGI:4822688
Gene symbol:	Pclaf
Gene name:	PCNA clamp associated factor

Probe:	ES470 probe 437
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing. A tyramide-biotin/streptavidin amplification step was included in the in situ hybridization procedure.

Results

euxassay_002064_01 ©

Specimen euxassay_002064_01: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_01

euxassay_002064_02 ©

Specimen euxassay_002064_02: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_02

euxassay_002064_03 ©

Specimen euxassay_002064_03: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_03

euxassay_002064_04 ©

Specimen euxassay_002064_04: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_04

euxassay_002064_05 ©

Specimen euxassay_002064_05: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_05

euxassay_002064_06 ©

Specimen euxassay_002064_06: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_06

euxassay_002064_07 ©

Specimen euxassay_002064_07: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_07
TS23: thymus primordium	Moderate	Uniform	euxassay_002064_07

euxassay_002064_08 ©

Specimen euxassay_002064_08: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_08
TS23: thymus primordium	Strong	Uniform	euxassay_002064_08

euxassay_002064_09 ©

Specimen euxassay_002064_09: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_09
TS23: olfactory cortex ventricular layer	Strong	Uniform	euxassay_002064_09
TS23: thymus primordium	Strong	Uniform	euxassay_002064_09

euxassay_002064_10 ©

Specimen euxassay_002064_10: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: olfactory cortex ventricular layer	Strong	Uniform	euxassay_002064_10
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_10

euxassay_002064_11 ©

Specimen euxassay_002064_11: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_11
TS23: olfactory cortex ventricular layer	Strong	Uniform	euxassay_002064_11

euxassay_002064_12 ©

Specimen euxassay_002064_12: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_12

Specimen euxassay_002064_13: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_13

Specimen euxassay_002064_14: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_14

Specimen euxassay_002064_15: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_15

Specimen euxassay_002064_16: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_16

Specimen euxassay_002064_17: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong	Uniform	euxassay_002064_17

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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