Helt Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:89485 Nakatani T, et al., Helt, a novel basic-helix-loop-helix transcriptional repressor expressed in the developing central nervous system. J Biol Chem. 2004 Apr 16;279(16):16356-67
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:4820625
Gene symbol:	Helt
Gene name:	helt bHLH transcription factor

Antibody:	anti-Helt
Detection system:	Secondary antibody coupled to fluorescein

Results

8F ©

Specimen 8F: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain ventricular layer	Present	Graded	8F	Mostly ventral expression, some dorsal in mitotically active neuronal progenitors.

8G ©

Specimen 8G: embryonic day 12.5 (more

)
Note: Double labeled: green - Helt1; red - Tubb3 (assay MGI:4820624).

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		8G	Helt was not coexpressed with Tubb3 and is expressed in mitotically active neuronal progenitors.

8H ©

Specimen 8H: embryonic day 12.5 (more

)
Note: Double labeled: green - Helt1; red - Lhx1 (assay MGI:4820561).

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		8H	Helt was not coexpressed with Lim1 and is expressed in mitotically active neuronal progenitors.

8I ©

Specimen 8I: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		8I	Expression is in mitotically active neuronal progenitors.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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