Probe:	Lrrc14-P1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The probe used for this assay recognizes isoforms 1, 2 and 3.

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Ubiquitous	4A	Expression was ubiquitous throughout both neural and non-neural tissues.

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Present		4B
TS16: branchial arch	Weak		4B
TS16: limb	Weak		4B

Structure	Level	Pattern	Image	Note
TS18: forebrain	Present		4D
TS18: telencephalon	Present	Regionally restricted	4D	Expression was prominent in the olfactory bulbs.
TS18: future spinal cord	Present		4D
TS18: hindbrain	Present		4D
TS18: midbrain	Present		4D
TS18: midbrain-hindbrain junction	Present		4D
TS18: otocyst	Present		4D
TS18: head	Weak	Regionally restricted	4D	Expression was detected around the snout and in the facial processes.
TS18: limb	Weak		4D

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present		4E
TS21: olfactory lobe	Present	Regionally restricted	4E	Expression was prominent in the olfactory bulbs.
TS21: hindbrain	Present		4E
TS21: midbrain	Present		4E
TS21: midbrain-hindbrain junction	Present		4E
TS21: spinal cord	Present		4E
TS21: inner ear	Present	Regionally restricted	4E	Expression was detected in the otic vesicles.
TS21: head	Weak	Regionally restricted	4E	Expression was detected around the snout and the facial processes.
TS21: limb	Weak		4E

Structure	Level	Pattern	Image	Note
TS23: forebrain	Present		4F
TS23: hindbrain	Present		4F
TS23: main olfactory bulb	Present		4F
TS23: midbrain	Present		4F
TS23: midbrain-hindbrain junction	Present		4F
TS23: spinal cord	Present		4F
TS23: inner ear	Present	Regionally restricted	4F	Expression was detected in the otic vesicles.
TS23: head	Weak	Regionally restricted	4F	Expression was detected around the snout and in the facial processes.
TS23: limb	Weak		4F

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		4G
TS20: spinal cord	Present		4G

Structure	Level	Pattern	Image	Note
TS26: lateral ganglionic eminence	Weak		4I	Expression was greatly reduced in the medial and lateral ganglionic eminences.
TS26: medial ganglionic eminence	Weak		4I	Expression was greatly reduced in the medial and lateral ganglionic eminences.
TS26: cortical plate	Strong		4I , 4K
TS26: piriform cortex	Strong		4I , 4K
TS26: thalamus	Strong		4I , 4K
TS26: telencephalon	Strong	Regionally restricted	4I	Expression was strong in cells throughout the basal telencephalon.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present	Widespread	4J
TS22: cerebral cortex subventricular zone	Present		4J
TS22: piriform cortex	Present		4M , 4J
TS22: corpus striatum	Present	Regionally restricted	4M , 4J	Expression was detected in cells of the striatal subventricular zone and the putamen. No expression was seen in the cells of the striatal ventricular zone.
TS22: cerebral cortex ventricular layer	Present	Scattered	4J
TS22: cerebral cortex intermediate zone	Absent		4M , 4J
TS22: lateral ganglionic eminence	Strong		4J	Strong expression was seen in cells throughout the medial and lateral ganglionic eminences.
TS22: medial ganglionic eminence	Strong		4J	Strong expression was seen in cells throughout the medial and lateral ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Present		4N

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	5A	Expression was detected in cells throughout the cortical layers.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	5B , 5F	Expression was detected in cells throughout the cortical layers. Expression was also seen in the reticular granular and agranular cortex.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong		5C
TS28: hippocampus CA1	Strong		5C
TS28: hippocampus CA2	Strong		5C
TS28: hippocampus CA3	Strong		5C
TS28: thalamus	Present	Regionally restricted	5C	Expression was detected in cells of the thalamic nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	5D	Expression was detected in cells throughout the cortical layers.
TS28: dentate gyrus granule cell layer	Strong		5D
TS28: fasciola cinereum	Strong		5D
TS28: hippocampus CA1	Strong		5D
TS28: hippocampus CA2	Strong		5D
TS28: hippocampus CA3	Strong		5D
TS28: thalamus	Present	Regionally restricted	5D	Expression was detected in cells of the thalamic nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	5E	Expression was detected in cells throughout the cortical layers.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong		5G
TS28: hippocampus CA1	Strong		5G
TS28: hippocampus CA2	Strong		5G
TS28: hippocampus CA3	Strong		5G

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong		5H
TS28: hippocampus CA1	Strong		5H
TS28: hippocampus CA2	Strong		5H
TS28: hippocampus CA3	Strong		5H

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		5I
TS28: cerebellum molecular layer	Present	Scattered	5I
TS28: cerebellum Purkinje cell layer	Strong		5I

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present	Scattered	5J
TS28: cerebellum Purkinje cell layer	Strong		5J
TS28: raphe nucleus	Present	Regionally restricted	5J	Expression was detected in pyramidal cells in the raphe nucleus.

Structure	Level	Pattern	Image	Note
TS28: raphe nucleus	Present	Regionally restricted	5K	Expression was detected in pyramidal cells in the raphe nucleus.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		5L