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Gene Expression Data
Assay Details
Assay
Reference: J:82311 Yamamoto M, et al., Nodal signaling induces the midline barrier by activating Nodal expression in the lateral plate. Development. 2003 May;130(9):1795-804
Assay type: Recombinase reporter
MGI Accession ID: MGI:4459832
Gene symbol: Tg(Leftb-cre)1Hmd
Gene name: transgene insertion 1, Hiroshi Hamada
Reporter: lacZ
Direct Detection
Results
Specimen Fig. 1a: embryonic day 7.5; Tg(CAG-cat,-lacZ)11Miya/0, Tg(Leftb-cre)1Hmd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: embryo mesenchyme Present Widespread 1A Staining is observed in most of the mesoderm of the embryonic region.
TS11: embryo ectoderm Absent 1A
TS11: embryo endoderm Absent 1A
TS11: primitive streak Absent 1A

Specimen Fig. 1b: embryonic day 8.2; Tg(CAG-cat,-lacZ)11Miya/0, Tg(Leftb-cre)1Hmd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS12: embryo mesenchyme Present Regionally restricted 1B Staining is absent from midline structures like the axial mesoderm.
TS12: lateral plate mesoderm Present Regionally restricted 1B Staining is observed in the anterior region of the lateral plate mesoderm, but is absent in the prospective floorplate.
TS12: paraxial mesenchyme Present 1B

Specimen Fig. 1c: embryonic day 8.2; Tg(CAG-cat,-lacZ)11Miya/0, Tg(Leftb-cre)1Hmd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS12: embryo Present Non-Uniform 1C Staining pattern differs between the anterior and posterior regions ofthe embryo.
TS12: lateral plate mesoderm Present Regionally restricted 1C Staining observed in anterior region of LPM is complete. Posterior to the node, staining is observed only in the most lateral regions.
TS12: paraxial mesenchyme Present 1C Staining observed in anterior region of the paraxial mesoderm is complete.
TS12: embryo endoderm Present 1C Staining observed in anterior region of the definitive endoderm is complete.
TS12: heart Present 1C

Specimen not shown: embryonic day 8.2; Tg(CAG-cat,-lacZ)11Miya/0, Tg(Leftb-cre)1Hmd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS12: yolk sac Present Staining is observed in about 30-50% of extra-embryonic mesoderm cells.
TS12: amnion Present Staining is observed in about 30-50% of extra-embryonic mesoderm cells.

Specimen not shown: embryonic day 9.5; Tg(CAG-cat,-lacZ)11Miya/0, Tg(Leftb-cre)1Hmd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: embryo Present Regionally restricted 1C Staining is observed in mesoderm-derived cells in the anterior region, but mesoderm is posterior region is negative.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory