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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450880
Gene symbol: Nos1
Gene name: nitric oxide synthase 1, neuronal
Probe: UI-M-CG0p-brp-g-05-0-UI.s1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Results
Specimen Nos1 - E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Weak Non-Uniform Nos1-Shh-E11.5_sagittal , Nos1-Shh E11.5_coronal There is weak staining in the MZ of the premam zone. Staining is either absent or ubiquitous in the MZ of the dorsal thalamus, prethalamus, dorsal & ventral POAs, the PVH, VMH & ARH nuclei, the AVH, anterior & posterior IDs, mam & supramam zones & the TT.
TS19: diencephalon lateral wall ventricular layer Ambiguous Nos1-Shh-E11.5_sagittal , Nos1-Shh E11.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer Ambiguous Nos1-Shh E11.5_coronal , Nos1-Shh-E11.5_sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Ambiguous Nos1-Shh E11.5_coronal , Nos1-Shh-E11.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Specimen Nos1 - E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous Nos1-Shh E12.5_coronal , Nos1-Shh-E12.5_sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Ambiguous Nos1-Shh-E12.5_sagittal , Nos1-Shh E12.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous Nos1-Shh-E12.5_sagittal , Nos1-Shh E12.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Moderate Non-Uniform Nos1-Shh E12.5_coronal , Nos1-Shh-E12.5_sagittal There is moderate staining in the mantle zone of the premam zone and weak staining in the supramam zone. Staining is either absent or ubiquitous in the mantle zone of the TT and the mam zone.
TS20: diencephalon ventricular layer Ambiguous Nos1-Shh-E12.5_sagittal , Nos1-Shh E12.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Ambiguous Nos1-Shh-E12.5_sagittal , Nos1-Shh E12.5_coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer Ambiguous Nos1-Shh-E12.5_sagittal , Nos1-Shh E12.5_coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Ambiguous Nos1-Shh E12.5_coronal , Nos1-Shh-E12.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer Ambiguous Nos1-Shh-E12.5_sagittal , Nos1-Shh E12.5_coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer Ambiguous Nos1-Shh-E12.5_sagittal , Nos1-Shh E12.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Nos1 - E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Very strong Nos1-Shh E14.5_coronal , Nos1-Shh-E14.5_sagittal There is very strong staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Ambiguous Nos1-Shh-E14.5_sagittal , Nos1-Shh E14.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate Very strong Nos1-Shh E14.5_coronal , Nos1-Shh-E14.5_sagittal There is very strong staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Strong Non-Uniform Nos1-Shh E14.5_coronal , Nos1-Shh-E14.5_sagittal There is strong staining in the mantle zone of the premam zone. Staining is either absent or ubiquitous in the mantle zone of the mam and supramam zones and the TT.
TS22: diencephalon ventricular layer Ambiguous Nos1-Shh-E14.5_sagittal , Nos1-Shh E14.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence Ambiguous Nos1-Shh-E14.5_sagittal , Nos1-Shh E14.5_coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer Moderate Non-Uniform Nos1-Shh-E14.5_sagittal , Nos1-Shh E14.5_coronal There is moderate staining in the mantle zone of the PVH nucleus. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Ambiguous Nos1-Shh-E14.5_sagittal , Nos1-Shh E14.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer Very strong Non-Uniform Nos1-Shh E14.5_coronal , Nos1-Shh-E14.5_sagittal There is very strong staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS22: thalamus ventricular layer Ambiguous Nos1-Shh-E14.5_sagittal , Nos1-Shh E14.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Nos1 - E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Very strong Scattered Nos1-Shh-E16.5_sagittal , Nos1-Shh E16.5_coronal There is very strong, scattered staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Ambiguous Nos1-Shh-E16.5_sagittal , Nos1-Shh E16.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate Very strong Scattered Nos1-Shh E16.5_coronal , Nos1-Shh-E16.5_sagittal There is very strong, scattered staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Strong Non-Uniform Nos1-Shh-E16.5_sagittal , Nos1-Shh E16.5_coronal There is strong staining in the mantle zone of the premam zone and moderate staining in the mam zone. Staining is either absent or ubiquitous in the mantle zone of the supramam zone.
TS24: diencephalon ventricular layer Ambiguous Nos1-Shh E16.5_coronal , Nos1-Shh-E16.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence Strong Non-Uniform Nos1-Shh-E16.5_sagittal , Nos1-Shh E16.5_coronal There is strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS24: hypothalamus mantle layer Very strong Non-Uniform Nos1-Shh-E16.5_sagittal , Nos1-Shh E16.5_coronal There is very strong staining in the MZ of the PVH nucleus, strong staining in the dorsal and ventral POAs and moderate staining in the PH. Staining is either absent or ubiquitous in the MZ of the AVH, the DAH and the SCH, DMH, VMH and ARH nuclei.
TS24: hypothalamus ventricular layer Ambiguous Nos1-Shh E16.5_coronal , Nos1-Shh-E16.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Very strong Nos1-Shh-E16.5_sagittal , Nos1-Shh E16.5_coronal There is very strong staining in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer Ambiguous Nos1-Shh-E16.5_sagittal , Nos1-Shh E16.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.


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last database update
06/12/2024
MGI 6.13
The Jackson Laboratory