Probe:	UI-M-CG0p-bgw-h-05-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Ambiguous		Igfbp2-Shh E11.5-coronal , Igfbp2-Shh E11.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer	Strong	Non-Uniform	Igfbp2-Shh E11.5-sagittal , Igfbp2-Shh E11.5-coronal	Staining is strong in the PVH & VMH nuclei & premam & mam zones, strong in dorsal & ventral POAs, ARH nucleus & AVH, moderate in dorsal thalamus & prethalamus & absent or ubiquitous in anterior & posterior IDs, TT & supramam zone; some is scattered.
TS19: telencephalon mantle layer	Ambiguous		Igfbp2-Shh E11.5-coronal , Igfbp2-Shh E11.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Moderate	Non-Uniform	Igfbp2-Shh E11.5-sagittal , Igfbp2-Shh E11.5-coronal	There is moderate, scattered staining in the ventricular zone of the cerebral cortex. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Igfbp2-Shh E12.5-coronal , Igfbp2-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Very strong		Igfbp2-Shh E12.5-coronal , Igfbp2-Shh E12.5-sagittal	There is very strong staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Igfbp2-Shh E12.5-sagittal , Igfbp2-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Weak	Non-Uniform	Igfbp2-Shh E12.5-sagittal , Igfbp2-Shh E12.5-coronal	There is weak, scattered staining in the mantle zone of the mam and supramam zones. Staining is either absent or ubiquitous in the mantle zone of the premam zone and the TT.
TS20: diencephalon ventricular layer	Strong	Non-Uniform	Igfbp2-Shh E12.5-sagittal , Igfbp2-Shh E12.5-coronal	There is strong staining in the ventricular zone of the premam and mam zones and weak, scattered staining in the TT. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS20: ganglionic eminence	Weak	Scattered	Igfbp2-Shh E12.5-coronal , Igfbp2-Shh E12.5-sagittal	There is weak, scattered staining in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer	Moderate	Non-Uniform	Igfbp2-Shh E12.5-sagittal , Igfbp2-Shh E12.5-coronal	There is moderate, scattered staining in the mantle zone of the AVH. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Strong	Non-Uniform	Igfbp2-Shh E12.5-sagittal , Igfbp2-Shh E12.5-coronal	Staining is strong in the VZ of the ventral POA & VMH nucleus, strong & scattered in the ARH nucleus & weak & scattered in the dorsal POA & anterior & posterior IDs. Staining is either absent or ubiquitous in the VZ of the PVH nucleus & the AVH.
TS20: thalamus mantle layer	Moderate	Non-Uniform	Igfbp2-Shh E12.5-coronal , Igfbp2-Shh E12.5-sagittal	There is moderate, scattered staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus.
TS20: thalamus ventricular layer	Moderate	Non-Uniform	Igfbp2-Shh E12.5-coronal , Igfbp2-Shh E12.5-sagittal	There is moderate, scattered staining in the ventricular zone of the dorsal thalamus and weak, scattered staining in the prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Moderate	Scattered	Igfbp2-Shh E14.5-sagittal , Igfbp2-Shh E14.5-coronal	There is moderate, scattered staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Strong		Igfbp2-Shh E14.5-sagittal , Igfbp2-Shh E14.5-coronal	There is strong staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Moderate	Scattered	Igfbp2-Shh E14.5-sagittal , Igfbp2-Shh E14.5-coronal	There is moderate, scattered staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Ambiguous		Igfbp2-Shh E14.5-sagittal , Igfbp2-Shh E14.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Strong	Non-Uniform	Igfbp2-Shh E14.5-sagittal , Igfbp2-Shh E14.5-coronal	There is strong staining in the ventricular zone of the mam zone. Staining is either absent or ubiquitous in the ventricular zone of the premam and supramam zones and the TT.
TS22: ganglionic eminence	Weak	Scattered	Igfbp2-Shh E14.5-coronal , Igfbp2-Shh E14.5-sagittal	There is weak, scattered staining in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer	Ambiguous		Igfbp2-Shh E14.5-sagittal , Igfbp2-Shh E14.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer	Strong	Non-Uniform	Igfbp2-Shh E14.5-coronal , Igfbp2-Shh E14.5-sagittal	There is strong staining in the VZ of the ARH nucleus and moderate staining in the ventral POA and the AVH. Staining is either absent or ubiquitous in the VZ of the dorsal POA, the PVH and VMH nuclei and anterior and posterior IDs.
TS22: thalamus mantle layer	Weak	Non-Uniform	Igfbp2-Shh E14.5-coronal , Igfbp2-Shh E14.5-sagittal	There is weak staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus.
TS22: thalamus ventricular layer	Strong		Igfbp2-Shh E14.5-sagittal , Igfbp2-Shh E14.5-coronal	There is strong staining in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Igfbp2-Shh E16.5-sagittal , Igfbp2-Shh E16.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Very strong		Igfbp2-Shh E16.5-coronal , Igfbp2-Shh E16.5-sagittal	There is very strong staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Igfbp2-Shh E16.5-coronal , Igfbp2-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Weak	Non-Uniform	Igfbp2-Shh E16.5-coronal , Igfbp2-Shh E16.5-sagittal	There is weak, scattered staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones.
TS24: diencephalon ventricular layer	Very strong	Non-Uniform	Igfbp2-Shh E16.5-sagittal , Igfbp2-Shh E16.5-coronal	There is very strong staining in the ventricular zone of the mam zone. Staining is either absent or ubiquitous in the ventricular zone of the premam and supramam zones.
TS24: ganglionic eminence	Ambiguous		Igfbp2-Shh E16.5-sagittal , Igfbp2-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer	Ambiguous		Igfbp2-Shh E16.5-coronal , Igfbp2-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer	Strong	Non-Uniform	Igfbp2-Shh E16.5-coronal , Igfbp2-Shh E16.5-sagittal	There is strong staining in the VZ of the ARH nucleus and moderate staining in the ventral POA and the AVH. Staining is either absent or ubiquitous in the VZ of the dorsal POA, the PVH, SCH, DMH and VMH nuclei, the DAH and the PH.
TS24: thalamus mantle layer	Ambiguous		Igfbp2-Shh E16.5-sagittal , Igfbp2-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Very strong		Igfbp2-Shh E16.5-sagittal , Igfbp2-Shh E16.5-coronal	There is very strong staining in the ventricular zone of the dorsal thalamus and prethalamus.