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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450710
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Probe: UI-M-BH2.1-apx-e-01-0-UI.s1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Results
Specimen Isl1 - E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Very strong Non-Uniform Isl1-Shh E11.5-sagittal Staining is very strong in the MZ of the prethalamus & dorsal & ventral POAs, strong in anterior & posterior IDs, VMH & ARH nuclei, premam zone & TT & either absent or ubiquitous in the dorsal thalamus, PVH nucleus, AVH & mam & supramam zones.
TS19: diencephalon lateral wall ventricular layer Ambiguous Isl1-Shh E11.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer Very strong Non-Uniform Isl1-Shh E11.5-sagittal There is very strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex.
TS19: telencephalon ventricular layer Ambiguous Isl1-Shh E11.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Specimen Isl1 - E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous Isl1-Shh E12.5-sagittal , Isl1-Shh E12.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Ambiguous Isl1-Shh E12.5-coronal , Isl1-Shh E12.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous Isl1-Shh E12.5-sagittal , Isl1-Shh E12.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Strong Non-Uniform Isl1-Shh E12.5-coronal , Isl1-Shh E12.5-sagittal There is strong staining in the mantle zone of the premam zone and the TT. Staining is either absent or ubiquitous in the mantle zone of the mam and supramam zone.
TS20: diencephalon ventricular layer Ambiguous Isl1-Shh E12.5-sagittal , Isl1-Shh E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Strong Non-Uniform Isl1-Shh E12.5-coronal , Isl1-Shh E12.5-sagittal There is strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS20: hypothalamus mantle layer Strong Non-Uniform Isl1-Shh E12.5-sagittal , Isl1-Shh E12.5-coronal There is strong staining in the mantle zone of the dorsal and ventral POAs, the VMH and ARH nuclei and anterior and posterior IDs. Staining is either absent or ubiquitous in the mantle zone of the PVH nucleus and the AVH.
TS20: hypothalamus ventricular layer Ambiguous Isl1-Shh E12.5-coronal , Isl1-Shh E12.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer Very strong Non-Uniform Isl1-Shh E12.5-coronal , Isl1-Shh E12.5-sagittal There is very strong staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS20: thalamus ventricular layer Ambiguous Isl1-Shh E12.5-sagittal , Isl1-Shh E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Isl1 - E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Ambiguous Isl1-Shh E14.5-coronal , Isl1-Shh E14.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Ambiguous Isl1-Shh E14.5-coronal , Isl1-Shh E14.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate Ambiguous Isl1-Shh E14.5-sagittal , Isl1-Shh E14.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Strong Non-Uniform Isl1-Shh E14.5-sagittal , Isl1-Shh E14.5-coronal There is strong staining in the mantle zone of the premam zone and the TT. Staining is either absent or ubiquitous in the mantle zone of the mam and supramam zone.
TS22: diencephalon ventricular layer Ambiguous Isl1-Shh E14.5-coronal , Isl1-Shh E14.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence Very strong Non-Uniform Isl1-Shh E14.5-coronal , Isl1-Shh E14.5-sagittal There is very strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS22: hypothalamus mantle layer Strong Non-Uniform Isl1-Shh E14.5-sagittal , Isl1-Shh E14.5-coronal There is strong staining in the mantle zone of the AVH and VMH and ARH nuclei and moderate staining in the dorsal and ventral POAs. Staining is either absent or ubiquitous in the mantle zone of the PVH nucleus and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Strong Non-Uniform Isl1-Shh E14.5-sagittal , Isl1-Shh E14.5-coronal There is strong staining in the ventricular zone of the anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei and the AVH.
TS22: thalamus mantle layer Very strong Non-Uniform Isl1-Shh E14.5-sagittal , Isl1-Shh E14.5-coronal There is very strong staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS22: thalamus ventricular layer Ambiguous Isl1-Shh E14.5-coronal , Isl1-Shh E14.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Isl1 - E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Ambiguous Isl1-Shh E16.5-coronal , Isl1-Shh E16.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Ambiguous Isl1-Shh E16.5-sagittal , Isl1-Shh E16.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate Ambiguous Isl1-Shh E16.5-coronal , Isl1-Shh E16.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Strong Non-Uniform Isl1-Shh E16.5-sagittal , Isl1-Shh E16.5-coronal There is strong staining in the mantle zone of the premam zone. Staining is either absent or ubiquitous in the mantle zone of the mam and supramam zones.
TS24: diencephalon ventricular layer Ambiguous Isl1-Shh E16.5-sagittal , Isl1-Shh E16.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence Very strong Non-Uniform Isl1-Shh E16.5-coronal , Isl1-Shh E16.5-sagittal There is very strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS24: hypothalamus mantle layer Strong Non-Uniform Isl1-Shh E16.5-coronal , Isl1-Shh E16.5-sagittal Staining is strong in the MZ of the AVH, DAH & PH, strong & scattered in the DMH, VMH & ARH nuclei, moderate in the dorsal POA & weak & scattered in the ventral POA. Staining is either absent or ubiquitous in the MZ of the PVH & SCH nuclei.
TS24: hypothalamus ventricular layer Ambiguous Isl1-Shh E16.5-coronal , Isl1-Shh E16.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Very strong Non-Uniform Isl1-Shh E16.5-coronal , Isl1-Shh E16.5-sagittal There is very strong staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS24: thalamus ventricular layer Ambiguous Isl1-Shh E16.5-coronal , Isl1-Shh E16.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.


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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory