Vegfa RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:27451 Breier G, et al., Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation. Development. 1992 Feb;114(2):521-32
Assay type:	RT-PCR
MGI Accession ID:	MGI:4443212
Gene symbol:	Vegfa
Gene name:	vascular endothelial growth factor A

Probe:	VEGF-pA1, VEGF-pB1
Assay notes:	PCR products were analyzed by Southern blot using the VEGF-1 cDNA (MGI:25266).

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	580 bp (b)	450 bp (c)	Amount	Genetic Background	Mutant Allele(s)	Sex
embryo 16d	E16.0 (a)	TS24: embryo	Present	Present (d)	0.1 µg; poly-A+ RNA	Not Specified		Not Specified
adult brain	P adult	TS28: brain	Present (e)	Present	0.1 µg; poly-A+ RNA	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) VEGF-1
(c) VEGF-2
(d) VEGF-2 transcripts were slightly more abundant than VEGF-1
(e) VEGF-1 transcripts were of highest abundance.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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