Gene Expression Data
Assay Details
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Assay
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Reference:
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J:23922
Parr BA, et al., Dorsalizing signal Wnt-7a required for normal polarity of D-V and A-P axes of mouse limb. Nature. 1995 Mar 23;374(6520):350-3
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4441506
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe62
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4c:
embryonic day 10.25
(more  )
Specimen
4c:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.25
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
4c
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Stronger expression in the dorsal half of the posterior mesenchyme.
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Specimen
4d:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 10.25
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Weak |
Regionally restricted |
4d
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Expression is substantially decreased throughout the zone of polarizing activity (ZPA) region.
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Specimen
4e:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 10.25
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Weak |
Regionally restricted |
4e
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Expression is substantially decreased throughout the zone of polarizing activity (ZPA) region.
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Analysis Tools
