Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:23922
Parr BA, et al., Dorsalizing signal Wnt-7a required for normal polarity of D-V and A-P axes of mouse limb. Nature. 1995 Mar 23;374(6520):350-3
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:4441504
|
|
Gene symbol:
|
Wnt7a
|
|
Gene name:
|
wingless-type MMTV integration site family, member 7A
|
|
Probe:
|
Wnt7a probe6
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
1a 8.75:
embryonic day 8.75
(more  )
Specimen
1a 8.75:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.75
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: forelimb bud ectoderm |
Present |
|
1a 8.75
|
Expression domain encompasses the presumptive forelimb region.
|
| TS14: surface ectoderm |
Present |
Regionally restricted |
1a 8.75
|
ExExpression extends from the level of the last few somites into the ectoderm overlying the presomitic mesoderm.
|
Specimen
1a 9.25:
embryonic day 9.25
(more )
Specimen
1a 9.25:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.25
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
|
1a 9.25
|
Expression in the ectoderm overlying the presumptive limb regions. Ectodermal expression first apparent at the level of the presumptive hindlimb.
|
| TS15: hindlimb ridge ectoderm |
Present |
|
1a 9.25
|
Expression in the ectoderm overlying the presumptive limb regions. Ectodermal expression first apparent at the level of the presumptive hindlimb.
|
Specimen
1a 10.5:
embryonic day 10.5
(more )
Specimen
1a 10.5:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb ectoderm |
Present |
Regionally restricted |
1a 10.5
|
Uniform expression throughout the dorsal limb ectoderm.
|
Specimen
1a 11.5:
embryonic day 11.5
(more )
Specimen
1a 11.5:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb ectoderm |
Present |
Regionally restricted |
1a 11.5
|
Uniform expression throughout the dorsal limb ectoderm.
|
|
Analysis Tools