Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:9026
Nomura S, et al., Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization. J Cell Biol. 1988 Feb;106(2):441-50
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:4441387
|
Gene symbol:
|
Sparc
|
Gene name:
|
secreted acidic cysteine rich glycoprotein
|
Probe:
|
Sparc cDNA4
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forelimb pre-cartilage condensation |
Present |
Regionally restricted |
|
Initially localized in a densely packed layer of cells around the outer surface of the cartilage shaft, in a region corresponding to the periosteum.
|
Specimen
3h,i:
embryonic day 14.5
(more )
Specimen
3h,i:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3k:
embryonic day 15.5
(more )
Specimen
3k:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: forelimb mesenchyme |
Strong |
Regionally restricted |
3k
|
High levels of expression in the outer periosteal layer, hypertrophic cartilage, and tendons.
|
Specimen
3m:
embryonic day 15.5
(more )
Specimen
3m:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: forelimb mesenchyme |
Present |
Regionally restricted |
3m
|
Expression in hypertrophic cartilage and cells in the outer periosteal layer.
|
Specimen
3o:
embryonic day 17.5
(more )
Specimen
3o:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: alveolar sulcus |
Present |
Regionally restricted |
3o
|
Expression in alveolar bone in the jaw in both periosteum and in cells on the surface of the internal bone trabeculae (endosteum).
|
|