Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:140465 Guo G, et al., Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. Dev Cell. 2010 Apr 20;18(4):675-85
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:4440941
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-Pou5f1
Detection system:	Secondary antibody
Assay notes:	Embryos were fixed in 2.5% paraformaldehyde.

Results

Specimen S1B -- second row: embryonic day 3.5 (more

)
Note: Double labeled: red - Pou5f1; green - Id2 (assay MGI:4440939).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		S1B Pou5f1 2nd row , S1B Merged 2nd row	Expression is present in the epiblast.

Specimen S1B -- fourth row: embryonic day 3.5 (more

)
Note: Double labeled: green - Pou5f1; red - Cebpa (assay MGI:4440942).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		S1B Pou5f1 4th row , S1B Merged 4th row	Expression is present in the epiblast.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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