Probe:	ngn1 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: neural crest	Absent		3A
TS13: neural tube	Absent		3A

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	3C	Strong expression in the ventricular zone of the ventral neural tube. Expression in a limited area of the dorsal neural tube near the roof plate.
TS17: dorsal root ganglion	Present	Regionally restricted	3C	Expression in numerous cells in the trunk dorsal root sensory ganglia.

Structure	Level	Pattern	Image	Note
TS17: enteric nervous system	Absent			No expression in sympathetic or enteric ganglia.
TS17: sympathetic nervous system	Absent			No expression in sympathetic or enteric ganglia.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	3E	Expression becomes restricted to the basal half of the neural tube and a narrow stripe adjacent to the roof plate.
TS20: spinal cord ventricular layer	Present	Regionally restricted	3E	Expression in a more lateral part of the ventricular zone.
TS20: dorsal root ganglion	Present	Regionally restricted	3E	Expression in trunk dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Absent			No expression in trunk dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Weak			Expression detectable in small numbers of cells in sensory ganglia and spinal cord.
TS24: spinal cord	Weak			Expression detectable in small numbers of cells in sensory ganglia and spinal cord.

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Present		5A	Expression in migrating neural crest at the both cervical and thoracolumbar levels.

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Present		5C	Expression in migrating neural crest at the both cervical and thoracolumbar levels.

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present		5E	Expression in forming sensory ganglia at both cervical and thoracolumbar levels.

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present		5G	Expression in forming sensory ganglia at both cervical and thoracolumbar levels.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		5I	Expression in dorsal root ganglia in the cervical region.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		5K	Expression in dorsal root ganglia at the level of the hindlimb.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Weak	Regionally restricted	6A	Weak expression in the cortical neuroepithelium.
TS20: forebrain ventricular layer	Present		6A	Expression in the forebrain, restricted to the ventricular zone.
TS20: diencephalon	Weak	Regionally restricted	6A	Weak expression in a portion of the diencephalon.
TS20: corpus striatum	Absent		6A

Structure	Level	Pattern	Image	Note
TS20: hindbrain ventricular layer	Present		6D

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted		Expression in medial areas of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	7A	No expression in lateral areas of the dorsal thalamus.
TS20: diencephalon	Present	Regionally restricted	7A	Expression in the subthalamus.
TS20: 4th ventricle	Present	Regionally restricted	7A	Expression in the neuroepithelium lining the lateral ventricle, aqueduct and 4th ventricle.
TS20: lateral ventricle	Present	Regionally restricted	7A	Expression in the neuroepithelium lining the lateral ventricle, aqueduct and 4th ventricle.
TS20: mesencephalic vesicle	Present	Regionally restricted	7A	Expression in the neuroepithelium lining the lateral ventricle, aqueduct and 4th ventricle.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	7C	Expression in the subthalamus.
TS20: mesencephalic vesicle	Present	Regionally restricted	7C	Expression in the neuroepithelium lining the aqueduct.
TS20: midbrain	Present	Regionally restricted	7C	No expression in the posterior tegmental epithelium.

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Present		8A

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present		8D

Structure	Level	Pattern	Image	Note
TS22: retina	Absent		8G

Structure	Level	Pattern	Image	Note
TS17: glossopharyngeal ganglion	Absent		8J

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present		8M
TS17: otocyst	Present		8M
TS17: facial ganglion	Absent		8M

Structure	Level	Pattern	Image	Note
TS20: diencephalon ventricular layer	Present	Regionally restricted		No expression in the ventricular zone dorsal to the pituitary.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Absent			No expression in the pancreatic primordium.