Gene Expression Data
Assay Details
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Assay
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Reference:
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J:156017
Yokoyama S, et al., A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58. Dev Cell. 2009 Dec;17(6):836-48
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4429970
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Gene symbol:
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Id2
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Gene name:
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inhibitor of DNA binding 2
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Antibody:
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anti-Id2
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Detection system:
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Secondary antibody
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Results
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Specimen
5:
embryonic day 10.5
(more )
Specimen
5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: somite |
Trace |
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5E E10.5 RP58+/+ Id2
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The authors did not specify the levels of expression in the wild type; it is possible there was none.
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Specimen
5:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Zbtb18tm1Haok/Zbtb18tm1Haok
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: somite |
Present |
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5E E10.5 RP58-/- Id2
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Expression is upregulated in mutants compared to wild type.
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Specimen
5:
embryonic day 13.5
(more )
Specimen
5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: myotome |
Trace |
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5E E13.5 Somite RP58+/+
,
5E E13.5 Somite RP58+/+ Id2
,
5E E13.5 Somite RP58+/+ Merge
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The authors did not specify the levels of expression in the wild type; it is possible there was none.
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Specimen
5:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Zbtb18tm1Haok/Zbtb18tm1Haok
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: myotome |
Present |
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5E E13.5 Somite RP58-/- Id2
,
5E E13.5 Somite RP58-/-
,
5E E13.5 Somite RP58-/- Merge
|
Expression is upregulated in mutants compared to wild type.
|
Specimen
5:
embryonic day 13.5
(more )
Specimen
5:
(close )
|
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindlimb musculature |
Trace |
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5E E13.5 Hindlimb RP58+/+ Merge
,
5E E13.5 Hindlimb RP58+/+
,
5E E13.5 Hindlimb RP58+/+ Id2
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The authors did not specify the levels of expression in the wild type; it is possible there was none.
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Specimen
5:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6
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Mutant Allele(s):
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Zbtb18tm1Haok/Zbtb18tm1Haok
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindlimb musculature |
Present |
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5E E13.5 Hindlimb RP58-/-
,
5E E13.5 Hindlimb RP58-/- Id2
,
5E E13.5 Hindlimb RP58-/- Merge
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Expression is upregulated in mutants compared to wild type.
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