Tcf4 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:156017 Yokoyama S, et al., A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58. Dev Cell. 2009 Dec;17(6):836-48
Assay type:	RNA in situ
MGI Accession ID:	MGI:4429948
Gene symbol:	Tcf4
Gene name:	transcription factor 4

Probe:	Tcf4 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

Specimen Table 1: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Strong			Expression is present in the muscle-forming region.

Specimen S2: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: limb mesenchyme	Absent		S2_Tcf4 E9.5	This annotation was inferred from Supplementary Table 4.

Specimen S2: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present		S2_Tcf4 E10.5	This annotation was inferred from Supplementary Table 4.

Specimen S2: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Present		S2_Tcf4 E11.5	Expression is present within the muscle-forming tissues in the limb middle mesenchyme.

Specimen Table S4 - E9.5: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: olfactory placode	Moderate
TS15: otic pit	Moderate

Specimen Table S4 - E10.5: embryonic day 10.5 (more

)

Structure	Level	Pattern	Note
TS17: limb mesenchyme	Strong	Non-Uniform	Staining is strong in the progress zone and proximal limb and moderate in the middle limb.
TS17: trunk somite	Strong
TS17: 1st branchial arch maxillary component	Strong		Staining is present in the maxillary process.
TS17: 1st branchial arch	Strong		Staining is present in the mandibular arch.
TS17: 2nd branchial arch	Moderate		Staining is present in the hyoid arch.
TS17: nasal pit	Moderate		Staining is present in the olfactory placode.
TS17: otocyst	Moderate		Staining is present in the otic pit.

Specimen Table S4 - E11.5: embryonic day 11.5 (more

)

Structure	Level	Pattern	Note
TS19: limb mesenchyme	Strong	Regionally restricted	Staining is present in the progress zone, middle and proximal limb and muscle.
TS19: trunk somite	Strong
TS19: maxillary process	Strong		Staining is present in the maxillary process.
TS19: 1st branchial arch	Strong		Staining is present in the mandibular arch.
TS19: 2nd branchial arch	Moderate		Staining is present in the hyoid arch.
TS19: nasal pit	Moderate		Staining is present in the olfactory placode.
TS19: inner ear	Moderate		Staining is present in the otic pit.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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