Probe:	Id3 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Strong			Expression is present in the muscle-forming region.

Structure	Level	Pattern	Image	Note
TS15: limb mesenchyme	Present		S2_Id3 E9.5	Expression is present in the muscle region.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present		S2_Id3 E10.5	Expression is present in the progress zone of the distal mesenchyme, as well as in the muscle region.

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Present		S2_Id3 E11.5	Expression is present in the progress zone of the distal mesenchyme, as well as in the muscle region.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Absent		S8_Id3 E12.5	There is no expression in muscle regions.
TS20: forelimb interdigital region	Present		S8_Id3 E12.5
TS20: hand	Present	Regionally restricted	S8_Id3 E12.5	Expression is present in distal autopod regions.

Structure	Level	Pattern	Image	Note
TS21: forelimb musculature	Absent		S8_Id3 E13.5	There is no expression in muscle regions.
TS21: forelimb interdigital region	Present		S8_Id3 E13.5
TS21: hand	Present	Regionally restricted	S8_Id3 E13.5	Expression is present in distal autopod regions.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud presumptive apical ectodermal ridge	Strong
TS15: limb mesenchyme	Strong	Regionally restricted		Staining is present in the distal limb.
TS15: limb	Strong			There is strong staining in the anterior and posterior limb.
TS15: trunk somite	Strong
TS15: heart	Moderate
TS15: 1st branchial arch maxillary component	Strong			Staining is present in the maxillary process.
TS15: 1st branchial arch	Moderate			Staining is present in the mandibular arch.
TS15: eye	Strong
TS15: genitourinary system	Moderate	Regionally restricted		Staining is present in the external genitalia.
TS15: telencephalon	Strong
TS15: diencephalon	Strong
TS15: future midbrain	Strong
TS15: future hindbrain	Strong			Staining is present in the metencephalon.
TS15: neural tube	Strong			There is strong staining in the medial and lateral neural tube.

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Strong
TS17: limb mesenchyme	Strong	Non-Uniform		Staining is strong in the progress zone and moderate in the middle limb.
TS17: limb	Strong			There is strong staining in the anterior and posterior limb.
TS17: trunk somite	Strong
TS17: heart	Moderate
TS17: liver	Strong
TS17: 1st branchial arch maxillary component	Strong			Staining is present in the maxillary process.
TS17: 1st branchial arch	Moderate			Staining is present in the mandibular arch.
TS17: eye	Strong
TS17: genitourinary system	Strong	Regionally restricted		Staining is present in the external genitalia.
TS17: otocyst	Strong			Staining is present in the otic pit.
TS17: midbrain	Strong
TS17: hindbrain	Strong			Staining is present in the metencephalon.
TS17: neural tube	Strong	Regionally restricted		Staining is present in the medial neural tube.

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Strong
TS19: limb mesenchyme	Strong	Non-Uniform		Staining is strong in the progress zone and muscle and moderate in the middle limb.
TS19: trunk somite	Strong
TS19: liver	Strong
TS19: eye	Strong
TS19: genital tubercle	Moderate			Staining is present in the external genitalia.
TS19: inner ear	Strong			Staining is present in the otic pit.
TS19: midbrain	Strong
TS19: hindbrain	Strong			Staining is present in the metencephalon.
TS19: neural tube	Strong	Regionally restricted		Staining is present in the medial neural tube.