Gene Expression Data
Assay Details
Assay
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Reference:
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J:154373
Town L, et al., The metalloendopeptidase gene Pitrm1 is regulated by hedgehog signaling in the developing mouse limb and is expressed in muscle progenitors. Dev Dyn. 2009 Oct 29;238(12):3175-3184
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4417963
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Probe:
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Pax3 cDNA10
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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At least three embryos were examined at each stage.
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Results
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Specimen
2K:
embryonic day 11.5
(more )
Specimen
2K:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2M:
embryonic day 11.5
(more )
Note: This embryo has 46 somites.
Specimen
2M:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This embryo has 46 somites.
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Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
2M
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Expression is present in two migratory streams of cells in the proximal limb; these cells are believed to be muscle cell progenitors.
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Specimen
2O:
embryonic day 11.5
(more )
Specimen
2O:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS19: trunk dermomyotome |
Present |
Regionally restricted |
2O
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Expression is present in the limb flank region in the lateral dermomyotome where hypaxial muscle progenitors originate.
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