Antibody:	Anti-Calpain 1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Slides were dried briefly and fixed in 10% formaldehyde including 1% t-octylphenoxypoly-ethoxyethanol.

Structure	Level	Pattern	Image	Note
TS22: molar enamel organ	Weak	Diffuse	2a

Structure	Level	Pattern	Image	Note
TS24: molar enamel organ	Weak	Diffuse	2b

Structure	Level	Pattern	Image	Note
TS26: molar enamel organ	Weak	Diffuse	2c

Structure	Level	Pattern	Image	Note
TS27: molar	Strong	Regionally restricted	2d	Expression was intense in ameloblasts.

Structure	Level	Pattern	Image	Note
TS28: molar	Present	Regionally restricted	2e	Expression was detected in dentin.

Structure	Level	Pattern	Image	Note
TS26: skin	Present	Regionally restricted	3Aa	Expression was detected in the skin. Staining was moderate in the basal layer, and weak in the peridermal layer.

Structure	Level	Pattern	Image	Note
TS26: molar enamel organ	Weak	Diffuse	3Ba	Expression was light and diffuse in the enamel epithelium.

Structure	Level	Pattern	Image	Note
TS27: enamel organ	Present	Regionally restricted	3Bd	Expression was detected in the enamel protein on the secretory side of the ameloblasts.

Structure	Level	Pattern	Image	Note
TS28: molar	Present	Regionally restricted	3Ca	Expression was detected in the dentin.

Structure	Level	Pattern	Image	Note
TS28: molar	Present	Regionally restricted	3Ca'	Expression was detected in the dentin.

Structure	Level	Pattern	Image	Note
TS22: molar dental lamina	Weak
TS22: oral epithelium	Weak
TS22: molar epithelium	Weak	Regionally restricted		Expression was weak in outer enamel epithelium.
TS22: stellate reticulum	Weak

Structure	Level	Pattern	Image	Note
TS24: molar dental lamina	Weak
TS24: oral epithelium	Weak
TS24: molar epithelium	Weak	Regionally restricted		Expression was weak in the outer enamel epithelium.
TS24: stellate reticulum	Weak

Structure	Level	Pattern	Image	Note
TS26: molar dental lamina	Moderate
TS26: oral epithelium	Moderate
TS26: molar epithelium	Weak	Regionally restricted		Expression was weak in outer enamel epithelium and inner enamel epithelium.
TS26: molar	Weak	Regionally restricted		Expression was weak in odontoblasts and extracellular (enamel, dentin) space.
TS26: stellate reticulum	Weak
TS26: stratum intermedium	Weak

Structure	Level	Pattern	Image	Note
TS27: dental lamina	Moderate
TS27: oral epithelium	Moderate
TS27: stratum intermedium	Moderate
TS27: molar	Present	Regionally restricted		Expression was strong in ameloblasts, and weak in outer enamel epithelium and odontoblasts.
TS27: stellate reticulum	Weak

Structure	Level	Pattern	Image	Note
TS28: dental lamina	Weak
TS28: oral epithelium	Weak
TS28: molar	Weak	Regionally restricted		Expression was weak in extracellular (enamel, dentin) space.