Probe:	Ack1/Py1k cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Strong		1A
TS22: neocortex	Strong		1A
TS22: thalamus	Strong		1A
TS22: ganglionic eminence	Strong	Regionally restricted	1A	Expression is in the rostral migratory pathway.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Strong		1B
TS26: dentate gyrus	Strong		1B
TS26: hippocampus	Strong		1B
TS26: thalamus	Strong		1B

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong		1C
TS28: thalamus	Strong		1C
TS28: hippocampus	Strong		1C	Expression is particularly strong in interneurons located parallel to the hippocampal fissure and in Mossy cells of hilus.
TS28: dentate gyrus	Strong		1C
TS28: hippocampus CA1	Strong		1C
TS28: hippocampus CA3	Strong		1C

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Present	Regionally restricted	1D	Expression is in the mitral cells and glomerular cells.

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present	Regionally restricted	1E	Expression is in the mitral cells and glomerular cells.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		1F
TS28: main olfactory bulb mitral cell layer	Present		1F

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong		1G
TS28: main olfactory bulb mitral cell layer	Strong		1G

Structure	Level	Pattern	Image	Note
TS20: cortical plate	Very strong		1H

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Very strong		1I

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Very strong		1J

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Very strong		1K
TS27: cortical layer III	Very strong		1K
TS27: cortical layer IV	Very strong		1K
TS27: cortical layer V	Very strong		1K
TS27: cortical layer VI	Very strong		1K

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Very strong		1L
TS28: cortical layer III	Very strong		1L
TS28: cortical layer IV	Very strong		1L
TS28: cortical layer VI	Very strong		1L
TS28: cortical layer V	Very strong		1L	Expression was stronger in layer V.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present		1M

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Present	Regionally restricted	1N	Expression is also in precursor cells of the dentate gyrus.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong		1O	Expression is particularly strong in interneurons located parallel to the hippocampal fissure and in Mossy cells of hilus.
TS27: dentate gyrus	Strong		1O
TS27: hippocampus CA1	Strong		1O
TS27: hippocampus CA3	Strong		1O

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong		1P	Expression is particularly strong in interneurons located parallel to the hippocampal fissure and in Mossy cells of hilus.
TS28: dentate gyrus	Strong		1P
TS28: hippocampus CA1	Strong		1P
TS28: hippocampus CA3	Strong		1P

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong		1Q	Expression is particularly strong in interneurons located parallel to the hippocampal fissure and in Mossy cells of hilus.
TS28: hippocampus CA3	Strong		1Q
TS28: dentate gyrus	Present		1Q
TS28: hippocampus CA1	Present		1Q

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong		1R	Expression is particularly strong in interneurons located parallel to the hippocampal fissure and in Mossy cells of hilus.
TS28: dentate gyrus	Strong		1R
TS28: hippocampus CA1	Strong		1R
TS28: hippocampus CA3	Strong		1R

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		1S	Expression is particularly strong in interneurons located parallel to the hippocampal fissure and in Mossy cells of hilus.
TS28: hippocampus CA1	Present		1S
TS28: dentate gyrus granule cell layer	Present	Regionally restricted	1S	Expression is in the subgranular zone.
TS28: hippocampus CA3	Strong		1S

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present		2A

Structure	Level	Pattern	Image	Note
TS24: thalamus	Present		2B

Structure	Level	Pattern	Image	Note
TS27: prethalamus	Strong		2C
TS27: hypothalamus	Strong	Regionally restricted	2C	Expression is dorsal.
TS27: hypothalamus lateral zone	Strong		2C
TS27: habenula	Weak		2C

Structure	Level	Pattern	Image	Note
TS28: prethalamus	Present		2D
TS28: lateral geniculate nucleus	Present		2D
TS28: hypothalamus	Present	Regionally restricted	2D	Expression is dorsal.
TS28: hypothalamus lateral zone	Strong		2D
TS28: habenula	Weak		2D

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Strong	Regionally restricted	2E	Expression is in the rostral migratory pathway.

Structure	Level	Pattern	Image	Note
TS24: diencephalon ventricular layer	Present		2F

Structure	Level	Pattern	Image	Note
TS27: brain subventricular zone	Present	Regionally restricted	2G
TS27: diencephalon	Present	Regionally restricted	2G

Structure	Level	Pattern	Image	Note
TS28: diencephalon	Present	Regionally restricted	2H

Structure	Level	Pattern	Image	Note
TS28: diencephalon	Present	Regionally restricted	2I

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present		2J

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present		2K

Structure	Level	Pattern	Image	Note
TS22: brainstem	Strong		3A	Expression is intense in the rhombic lip.
TS22: cerebellum	Strong		3A	Expression is intense in the rhombic lip.

Structure	Level	Pattern	Image	Note
TS26: brainstem	Present	Regionally restricted	3B	Expression is in the external granule layer and in the Purkinje layer.
TS26: cerebellum	Present	Regionally restricted	3B	Expression is in the external granule layer and in the Purkinje layer.
TS26: periaqueductal grey	Present		3B

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		3C
TS28: cerebellum Purkinje cell layer	Strong		3C

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		3D
TS28: cerebellum internal granule cell layer	Strong		3D

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		3E
TS28: cerebellum internal granule cell layer	Strong		3E

Structure	Level	Pattern	Image	Note
TS22: brainstem	Present	Regionally restricted	3F	Expression is in the floor plate and vestibulocochlear nuclei.

Structure	Level	Pattern	Image	Note
TS24: pons	Present	Regionally restricted	3G	Expression is in the pontine migratory stream and the spinotrigeminal nuclei.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	3H	Expression is in solitary, vestibular, and dorsal motor nuclei.

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Present		3I
TS28: gigantocellular reticular nucleus	Present		3I

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present		3J

Structure	Level	Pattern	Image	Note
TS24: medulla oblongata	Strong		3K

Structure	Level	Pattern	Image	Note
TS28: cuneate nucleus	Strong		3L
TS28: hypoglossal nucleus	Strong		3L
TS28: medulla oblongata	Strong		3L
TS28: solitary tract nucleus	Strong		3L
TS28: trigeminal V spinal sensory nucleus	Strong		3L

Structure	Level	Pattern	Image	Note
TS28: cuneate nucleus	Strong		3M
TS28: hypoglossal nucleus	Strong		3M
TS28: medulla oblongata	Strong		3M
TS28: solitary tract nucleus	Strong		3M
TS28: trigeminal V spinal sensory nucleus	Strong		3M

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Weak
TS17: hindbrain	Weak	Regionally restricted		Expression is in the rhombic lip.
TS17: brain	Weak	Regionally restricted		Expression is in the floor plate of the brain stem.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Weak	Regionally restricted		Expression is in the olfactory ventricle and the cingulate and retrospinal cortex.
TS20: cerebral cortex	Weak	Regionally restricted		Expression is in the preplate.
TS20: cerebral cortex ventricular layer	Weak
TS20: forebrain	Weak	Regionally restricted		Expression is in the hippocampus primordium and is very low in the fimbria/fornix. Expression is weak in medial septum, and very weak in the amygdaloid complex.
TS20: diencephalon	Strong	Regionally restricted		Expression is in the subcomissural organ.
TS20: thalamus	Weak	Non-Uniform		Expression is weak in the medial thalamus and is very weak in the dorsal, ventral, and lateral thalamus.
TS20: hypothalamus	Weak	Regionally restricted		Expression is in dorsal and arcuate nuclei.
TS20: cerebellum primordium	Weak	Regionally restricted		Expression is in the upper and lower rhombic lip and vey weak in deep nuclei.
TS20: brainstem	Weak	Regionally restricted		Expression is in the floor plate, facial nucleus, spinal trigeminal nucleus, reticular formation, and solitary nucleus.
TS20: ganglionic eminence	Moderate

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Strong	Non-Uniform		Expression is in the olfactory ventricle and mitral cell layer and is weak in the glomerular cell layer.
TS22: telencephalon	Weak	Regionally restricted		Expression is in the piriform cortex and the cingulate and retrospinal cortex.
TS22: hippocampus	Weak	Non-Uniform		Expression is very weak in the fimbria/fornix.
TS22: cerebral cortex ventricular layer	Weak
TS22: cortical plate	Weak
TS22: ganglionic eminence	Strong
TS22: diencephalon	Very strong	Regionally restricted		Expression is in the subcomissural organ.
TS22: basal forebrain	Moderate	Regionally restricted		Expression is moderate in the medial septum and amygdaloid complex, weak in caudate-putamen, globus pallidus, and the accumbens nucleus.
TS22: basal ganglia	Moderate	Regionally restricted		Expression is moderate in the medial septum and amygdaloid complex, weak in caudate-putamen, globus pallidus, and the accumbens nucleus.
TS22: hypothalamus	Strong	Regionally restricted		Expression is in the arcuate nuclei, moderate in the dorsal and paraventricular nuclei, and is very weak in the ventral nuclei.
TS22: cerebellum	Strong	Regionally restricted		Expression is in the upper rhombic lip, weak in the external germinative layer, and is very weak in the deep nuclei.
TS22: hindbrain	Strong	Regionally restricted		Expression is in the upper rhombic lip, weak in the external germinative layer, and is very weak in the deep nuclei.
TS22: brainstem	Strong	Regionally restricted		Expression is very strong the floor plate, strong in lower rhombic lip, pontine migratory stream, moderate in cochlear nuclei, weak in facial nucleus, spinal trigeminal nucleus, reticular formation, dorsal motor vagus, and in a few other nuclei.
TS22: cerebral cortex intermediate zone	Trace
TS22: cortical subplate	Trace
TS22: cerebral aqueduct	Trace	Regionally restricted		Expression is in the periaqueductal gray area.
TS22: midbrain	Weak	Non-Uniform		Expression is in the dorsal raphe, superior and inferior colliculus, and is very weak in the substantia nigra.
TS22: thalamus	Weak	Non-Uniform		Expression is weak in the medial thalamus and is very weak in the dorsal, ventral, lateral thalamus, and reticular nuclei, and is absent in the lateral geniculate nuclei.
TS22: cerebral cortex marginal layer	Absent
TS22: epithalamus	Absent

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Very strong	Non-Uniform		Expression is in the mitral layer, is strong in the olfactory ventricle and is weak in the glomerular layer.
TS24: diencephalon	Very strong	Regionally restricted		Expression is in the subcomissural organ.
TS24: cortical plate	Strong
TS24: piriform cortex	Strong
TS24: superior colliculus	Strong
TS24: hippocampus	Strong	Non-Uniform		Expression is moderate in the dentate gyrus and is very weak in the fimbria/fornix.
TS24: thalamus	Strong	Non-Uniform		Expression is in the lateral geniculate and reticulate nuclei, moderate in lateral and ventral thalamus, weak in medial and dorsal thalamus.
TS24: hypothalamus	Strong	Regionally restricted		Expression is in paraventricular nuclei and arcuate nuclei, moderate in dorsal nuclei, and weak in ventral nuclei.
TS24: cerebral aqueduct	Very strong	Regionally restricted		Expression is in the periaqueductal gray area.
TS24: cerebellum	Strong	Regionally restricted		Expression is in the upper rhombic lip and Purkinje layer, moderate in the deep nuclei and the external granular layer.
TS24: brainstem	Strong	Regionally restricted		Expression is in the floor plate and in numerous nuclei. Absent in inferior olive.
TS24: telencephalon	Moderate	Regionally restricted		Expression is in the cingulate and retrospinal cortex, and entorhinal cortex.
TS24: rostral migratory stream	Moderate	Regionally restricted		Expression in subventricular zone/rostral migratory stream.
TS24: telencephalon subventricular zone	Moderate	Regionally restricted		Expression in subventricular zone/rostral migratory stream.
TS24: caudate-putamen	Moderate
TS24: cortical subplate	Moderate
TS24: epithalamus	Moderate
TS24: basal ganglia	Moderate	Regionally restricted		Expression is moderate in the globus pallidus, amygdaloid complex, andaccumbens nucleus, and dorso-lateral septum, weak in medial septum and taenia tecta.
TS24: forebrain	Moderate	Regionally restricted		Expression is moderate in the globus pallidus, amygdaloid complex, andaccumbens nucleus, and dorso-lateral septum, weak in medial septum and taenia tecta.
TS24: midbrain	Moderate	Regionally restricted		Expression is in dorsal raphe and is weak in substantia nigra.
TS24: cerebral cortex ventricular layer	Weak
TS24: inferior colliculus	Weak
TS24: cerebral cortex intermediate zone	Trace
TS24: ganglionic eminence	Trace
TS24: cerebral cortex marginal layer	Absent

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Very strong	Regionally restricted		Expression is in the mitral cell layer, strong in the granular layer, and is weak in the glomerular layer.
TS26: caudate-putamen	Strong
TS26: cortical plate	Strong
TS26: dentate gyrus	Strong
TS26: hippocampus CA1	Strong
TS26: hippocampus CA2	Strong
TS26: periaqueductal grey	Strong
TS26: piriform cortex	Strong
TS26: superior colliculus	Strong
TS26: telencephalon	Strong	Regionally restricted		Expression is in the cingulate and retrospinal cortex, and entorhinal cortex.
TS26: rostral migratory stream	Strong	Regionally restricted		Expression in subventricular zone/rostral migratory stream.
TS26: telencephalon subventricular zone	Strong	Regionally restricted		Expression in subventricular zone/rostral migratory stream.
TS26: midbrain	Strong	Regionally restricted		Expression is in dorsal raphe and is weak in substantia nigra.
TS26: hippocampus	Strong	Regionally restricted		Expression is in the subiculum, very weak in the fimbria/fornix, and absent in alveus.
TS26: forebrain	Strong	Regionally restricted		Expression is in the dorso-lateral septum, taenia tecta, and amygdaloid complex, moderate in globus pallidus, and accumbens nucleus, weak in medial septum.
TS26: diencephalon	Strong	Regionally restricted		Expression is in the subcomissural organ.
TS26: thalamus	Strong	Non-Uniform		Expression is in the lateral geniculate, reticulate nuclei, and ventral thalamus, moderate in lateral, medial and dorsal thalamus.
TS26: hypothalamus	Strong	Regionally restricted		Expression is in paraventricular nuclei and arcuate nuclei, moderate in dorsal nuclei, and weak in ventral nuclei.
TS26: cortical subplate	Moderate
TS26: epithalamus	Moderate
TS26: hippocampus CA3	Moderate
TS26: cerebellum	Strong	Regionally restricted		Expression is strong in the Purkinje layer and moderate in the upper rhombic lip, the deep nuclei and the external granular layer.
TS26: brainstem	Strong	Regionally restricted		Expression is in the floor plate and in numerous nuclei.
TS26: brainstem nucleus	Strong	Regionally restricted		Expression is in the floor plate and in numerous nuclei.
TS26: cerebral cortex ventricular zone	Weak
TS26: inferior colliculus	Weak
TS26: cerebral cortex intermediate zone	Trace
TS26: cerebral cortex marginal zone	Absent

Structure	Level	Pattern	Image	Note
TS27: cerebellum internal granule cell layer	Very strong
TS27: hippocampus CA1	Very strong
TS27: hippocampus CA2	Very strong
TS27: lateral geniculate nucleus	Very strong
TS27: main olfactory bulb mitral cell layer	Very strong
TS27: neocortex layer	Strong	Regionally restricted		Expression is in the cortical plate, moderate in the subplate, weak in the ventricular zone.
TS27: thalamus	Strong	Regionally restricted		Strong to moderate expression in ventral, medial, and lateral thalamus.
TS27: amygdala	Strong
TS27: arcuate nucleus	Strong
TS27: cingulate cortex	Strong
TS27: cochlear nucleus	Strong
TS27: dentate gyrus	Strong
TS27: dorsal motor nucleus of vagus nerve	Strong
TS27: entorhinal cortex	Strong
TS27: periaqueductal grey	Strong
TS27: piriform cortex	Strong
TS27: reticular thalamic nucleus	Strong
TS27: solitary tract nucleus	Strong
TS27: subiculum	Strong
TS27: superior colliculus	Strong
TS27: taenia tecta	Strong
TS27: trigeminal V spinal sensory nucleus	Strong
TS27: brainstem	Strong	Regionally restricted		Expression is in floor plate and tegmental pontine nuclei.
TS27: accumbens nucleus	Moderate
TS27: caudate-putamen	Moderate
TS27: cerebellum external granule cell layer	Moderate
TS27: cuneate nucleus	Moderate
TS27: deep cerebellar nucleus	Moderate
TS27: dorsal raphe nucleus	Moderate
TS27: dorsal thalamus	Moderate
TS27: epithalamus	Moderate
TS27: facial nucleus	Moderate
TS27: globus pallidus	Moderate
TS27: habenula	Moderate
TS27: hippocampus CA3	Moderate
TS27: main olfactory bulb glomerular layer	Moderate
TS27: main olfactory bulb granule cell layer	Moderate
TS27: vestibular nucleus	Moderate
TS27: cerebral aqueduct	Moderate	Regionally restricted		Expression is in the subcomissural organ.
TS27: rostral migratory stream	Present	Regionally restricted		Expression is in the subventricular zone/rostral migratory stream.
TS27: telencephalon subventricular zone	Present	Regionally restricted		Expression is in the subventricular zone/rostral migratory stream.
TS27: telencephalon septum	Strong	Regionally restricted		Expression strong in dorso-lateral septum, weak in medial septum.
TS27: hypothalamic nucleus	Moderate	Regionally restricted		Moderate in dorsal nuclei, weak in ventral nuclei.
TS27: brainstem reticular formation	Weak
TS27: hypoglossal nucleus	Weak
TS27: inferior colliculus	Weak
TS27: inferior olivary nucleus	Weak
TS27: paraventricular hypothalamic nucleus	Weak
TS27: substantia nigra	Weak
TS27: pons	Weak	Regionally restricted		Expression is in the pontine migratory stream.
TS27: fimbria hippocampus	Trace
TS27: hippocampus	Trace	Regionally restricted		Expression is in the alveus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Very strong
TS28: dentate gyrus	Very strong
TS28: hippocampus CA1	Very strong
TS28: hippocampus CA2	Very strong
TS28: lateral geniculate nucleus	Very strong
TS28: main olfactory bulb mitral cell layer	Very strong
TS28: amygdala	Strong
TS28: arcuate nucleus	Strong
TS28: cingulate cortex	Strong
TS28: cochlear nucleus	Strong
TS28: cortical layer IV	Strong
TS28: cortical layer V	Strong
TS28: cortical layer VI	Strong
TS28: deep cerebellar nucleus	Strong
TS28: dorsal motor nucleus of vagus nerve	Strong
TS28: dorsal raphe nucleus	Strong
TS28: entorhinal cortex	Strong
TS28: hippocampus CA3	Strong
TS28: periaqueductal grey	Strong
TS28: piriform cortex	Strong
TS28: reticular thalamic nucleus	Strong
TS28: solitary tract nucleus	Strong
TS28: subiculum	Strong
TS28: superior colliculus	Strong
TS28: taenia tecta	Strong
TS28: trigeminal V spinal sensory nucleus	Strong
TS28: hippocampus	Strong	Regionally restricted		Expression is in interneurons, trace in alveus.
TS28: rostral migratory stream	Moderate	Regionally restricted		Expression is moderate in the subventricular zone/rostral migratory stream.
TS28: telencephalon subventricular zone	Moderate	Regionally restricted		Expression is moderate in the subventricular zone/rostral migratory stream.
TS28: telencephalon septum	Strong	Regionally restricted		Expression strong in dorso-lateral septum, moderate in medial septum.
TS28: thalamus	Strong	Regionally restricted		Strong to moderate expression in ventral, medial, and lateral thalamus.
TS28: hypothalamus	Strong	Regionally restricted		Moderate in dorsal nuclei, strong in ventral nuclei.
TS28: brainstem	Strong	Regionally restricted		Expression is in tegmental pontine nuclei.
TS28: accumbens nucleus	Moderate
TS28: brainstem reticular formation	Moderate
TS28: cerebellum external granule cell layer	Moderate
TS28: cortical layer II	Moderate
TS28: cortical layer III	Moderate
TS28: cuneate nucleus	Moderate
TS28: dorsal thalamus	Moderate
TS28: facial nucleus	Moderate
TS28: globus pallidus	Moderate
TS28: hypoglossal nucleus	Moderate
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb granule cell layer	Moderate
TS28: vestibular nucleus	Moderate
TS28: cerebral aqueduct	Moderate	Regionally restricted		Expression is in the subcomissural organ.
TS28: area postrema	Weak
TS28: caudate-putamen	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: epithalamus	Weak
TS28: inferior colliculus	Weak
TS28: inferior olivary nucleus	Weak
TS28: substantia nigra	Weak
TS28: cerebellum molecular layer	Trace
TS28: fimbria hippocampus	Trace
TS28: paraventricular hypothalamic nucleus	Trace

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Very strong
TS28: cingulate cortex	Very strong
TS28: cortical layer IV	Very strong
TS28: cortical layer V	Very strong
TS28: cortical layer VI	Very strong
TS28: dentate gyrus	Very strong
TS28: hippocampus CA1	Very strong
TS28: hippocampus CA2	Very strong
TS28: lateral geniculate nucleus	Very strong
TS28: main olfactory bulb mitral cell layer	Very strong
TS28: piriform cortex	Very strong
TS28: subiculum	Very strong
TS28: hippocampus	Very strong	Regionally restricted		Expression is in interneurons, trace in the alveus.
TS28: rostral migratory stream	Weak	Regionally restricted		Expression is weak in the subventricular zone/rostral migratory stream.
TS28: telencephalon subventricular zone	Weak	Regionally restricted		Expression is weak in the subventricular zone/rostral migratory stream.
TS28: telencephalon septum	Very strong	Regionally restricted		Expression ver strong in dorso-lateral septum; moderate in medial septum.
TS28: amygdala	Strong
TS28: brainstem reticular formation	Strong
TS28: cortical layer II	Strong
TS28: cortical layer III	Strong
TS28: cuneate nucleus	Strong
TS28: deep cerebellar nucleus	Strong
TS28: dorsal motor nucleus of vagus nerve	Strong
TS28: dorsal raphe nucleus	Strong
TS28: entorhinal cortex	Strong
TS28: facial nucleus	Strong
TS28: hippocampus CA3	Strong
TS28: hypoglossal nucleus	Strong
TS28: inferior colliculus	Strong
TS28: periaqueductal grey	Strong
TS28: solitary tract nucleus	Strong
TS28: superior colliculus	Strong
TS28: taenia tecta	Strong
TS28: trigeminal V spinal sensory nucleus	Strong
TS28: vestibular nucleus	Strong
TS28: thalamus	Strong	Non-Uniform		Strong to moderate expression in ventral, medial, and lateral thalamus.
TS28: hypothalamus	Strong	Regionally restricted		Expression is in dorsal nuclei and ventral nuclei.
TS28: brainstem	Strong	Regionally restricted		Expression is in tegmental pontine nuclei.
TS28: accumbens nucleus	Moderate
TS28: arcuate nucleus	Moderate
TS28: area postrema	Moderate
TS28: cerebellum external granule cell layer	Moderate
TS28: cerebellum internal granule cell layer	Moderate
TS28: cerebellum molecular layer	Moderate
TS28: cochlear nucleus	Moderate
TS28: dorsal thalamus	Moderate
TS28: globus pallidus	Moderate
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb granule cell layer	Moderate
TS28: reticular thalamic nucleus	Moderate
TS28: cerebral aqueduct	Moderate	Regionally restricted		Expression is in the subcomissural organ.
TS28: caudate-putamen	Weak
TS28: epithalamus	Weak
TS28: inferior olivary nucleus	Weak
TS28: substantia nigra	Weak
TS28: fimbria hippocampus	Trace
TS28: paraventricular hypothalamic nucleus	Trace

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Very strong
TS28: cingulate cortex	Very strong
TS28: cortical layer IV	Very strong
TS28: cortical layer V	Very strong
TS28: cortical layer VI	Very strong
TS28: deep cerebellar nucleus	Very strong
TS28: dentate gyrus	Very strong
TS28: hippocampus CA1	Very strong
TS28: hippocampus CA2	Very strong
TS28: lateral geniculate nucleus	Very strong
TS28: main olfactory bulb mitral cell layer	Very strong
TS28: piriform cortex	Very strong
TS28: subiculum	Very strong
TS28: vestibular nucleus	Very strong
TS28: hippocampus	Very strong	Regionally restricted		Expression is in interneurons, trace in the alveus.
TS28: rostral migratory stream	Moderate	Regionally restricted		Expression is moderate in the subventricular zone/rostral migratory stream.
TS28: telencephalon subventricular zone	Moderate	Regionally restricted		Expression is moderate in the subventricular zone/rostral migratory stream.
TS28: telencephalon septum	Very strong	Regionally restricted		Expression very strong in dorso-lateral septum, moderate in medial septum.
TS28: brainstem reticular formation	Strong
TS28: cortical layer II	Strong
TS28: cortical layer III	Strong
TS28: cuneate nucleus	Strong
TS28: dorsal motor nucleus of vagus nerve	Strong
TS28: dorsal raphe nucleus	Strong
TS28: entorhinal cortex	Strong
TS28: facial nucleus	Strong
TS28: hippocampus CA3	Strong
TS28: hypoglossal nucleus	Strong
TS28: inferior colliculus	Strong
TS28: periaqueductal grey	Strong
TS28: solitary tract nucleus	Strong
TS28: superior colliculus	Strong
TS28: taenia tecta	Strong
TS28: thalamus	Strong	Non-Uniform		Strong to moderate expression in ventral, medial, and lateral thalamus.
TS28: hypothalamus	Strong	Regionally restricted		Expression is in dorsal nuclei and ventral nuclei.
TS28: brainstem	Strong	Regionally restricted		Expression is in tegmental pontine nuclei.
TS28: accumbens nucleus	Moderate
TS28: amygdala	Moderate
TS28: arcuate nucleus	Moderate
TS28: area postrema	Moderate
TS28: cerebellum internal granule cell layer	Moderate
TS28: cerebellum molecular layer	Moderate
TS28: cochlear nucleus	Moderate
TS28: dorsal thalamus	Moderate
TS28: globus pallidus	Moderate
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb granule cell layer	Moderate
TS28: reticular thalamic nucleus	Moderate
TS28: substantia nigra	Moderate
TS28: trigeminal V spinal sensory nucleus	Moderate
TS28: cerebral aqueduct	Moderate	Regionally restricted		Expression is in the subcomissural organ.
TS28: caudate-putamen	Weak
TS28: epithalamus	Weak
TS28: inferior olivary nucleus	Weak
TS28: fimbria hippocampus	Trace
TS28: paraventricular hypothalamic nucleus	Trace

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Very strong
TS28: cortical layer IV	Very strong
TS28: cortical layer V	Very strong
TS28: cortical layer VI	Very strong
TS28: deep cerebellar nucleus	Very strong
TS28: facial nucleus	Very strong
TS28: hippocampus CA3	Very strong
TS28: main olfactory bulb mitral cell layer	Very strong
TS28: piriform cortex	Very strong
TS28: subiculum	Very strong
TS28: vestibular nucleus	Very strong
TS28: hippocampus	Very strong	Regionally restricted		Expression is in interneurons, trace in the alveus.
TS28: cerebellum Purkinje cell layer	Strong
TS28: cortical layer II	Strong
TS28: cortical layer III	Strong
TS28: cuneate nucleus	Strong
TS28: dentate gyrus	Strong
TS28: dorsal motor nucleus of vagus nerve	Strong
TS28: dorsal raphe nucleus	Strong
TS28: entorhinal cortex	Strong
TS28: hippocampus CA1	Strong
TS28: hippocampus CA2	Strong
TS28: hypoglossal nucleus	Strong
TS28: inferior colliculus	Strong
TS28: lateral geniculate nucleus	Strong
TS28: periaqueductal grey	Strong
TS28: solitary tract nucleus	Strong
TS28: superior colliculus	Strong
TS28: taenia tecta	Strong
TS28: rostral migratory stream	Moderate	Regionally restricted		Expression is moderate in the subventricular zone/rostral migratory stream.
TS28: telencephalon subventricular zone	Moderate	Regionally restricted		Expression is moderate in the subventricular zone/rostral migratory stream.
TS28: telencephalon septum	Very strong	Regionally restricted		Expression very strong in dorso-lateral septum, moderate in medial septum.
TS28: thalamus	Strong	Non-Uniform		Strong to moderate expression in ventral, medial, and lateral thalamus.
TS28: hypothalamus	Strong	Regionally restricted		Expression is in dorsal nuclei and moderate in ventral nuclei.
TS28: brainstem	Strong	Regionally restricted		Expression is in tegmental pontine nuclei.
TS28: accumbens nucleus	Moderate
TS28: amygdala	Moderate
TS28: arcuate nucleus	Moderate
TS28: area postrema	Moderate
TS28: brainstem reticular formation	Moderate
TS28: cerebellum internal granule cell layer	Moderate
TS28: cochlear nucleus	Moderate
TS28: dorsal thalamus	Moderate
TS28: epithalamus	Moderate
TS28: globus pallidus	Moderate
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb granule cell layer	Moderate
TS28: reticular thalamic nucleus	Moderate
TS28: substantia nigra	Moderate
TS28: trigeminal V spinal sensory nucleus	Moderate
TS28: caudate-putamen	Weak
TS28: inferior olivary nucleus	Weak
TS28: cerebral aqueduct	Weak	Regionally restricted		Expression is in the subcomissural organ.
TS28: cerebellum molecular layer	Trace
TS28: fimbria hippocampus	Trace
TS28: paraventricular hypothalamic nucleus	Trace

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Very strong
TS28: cortical layer IV	Very strong
TS28: cortical layer V	Very strong
TS28: cortical layer VI	Very strong
TS28: deep cerebellar nucleus	Very strong
TS28: facial nucleus	Very strong
TS28: hippocampus CA3	Very strong
TS28: main olfactory bulb mitral cell layer	Very strong
TS28: piriform cortex	Very strong
TS28: subiculum	Very strong
TS28: vestibular nucleus	Very strong
TS28: hippocampus	Very strong	Regionally restricted		Expression is in interneurons, trace in the alveus.
TS28: cerebellum Purkinje cell layer	Strong
TS28: cortical layer II	Strong
TS28: cortical layer III	Strong
TS28: cuneate nucleus	Strong
TS28: dentate gyrus	Strong
TS28: dorsal motor nucleus of vagus nerve	Strong
TS28: dorsal raphe nucleus	Strong
TS28: entorhinal cortex	Strong
TS28: hippocampus CA1	Strong
TS28: hippocampus CA2	Strong
TS28: hypoglossal nucleus	Strong
TS28: inferior colliculus	Strong
TS28: lateral geniculate nucleus	Strong
TS28: periaqueductal grey	Strong
TS28: solitary tract nucleus	Strong
TS28: superior colliculus	Strong
TS28: taenia tecta	Strong
TS28: rostral migratory stream	Strong	Regionally restricted		Expression is strong in the subventricular zone/rostral migratory stream.
TS28: telencephalon subventricular zone	Strong	Regionally restricted		Expression is strong in the subventricular zone/rostral migratory stream.
TS28: telencephalon septum	Very strong	Regionally restricted		Expression very strong in dorso-lateral septum; moderate in medial septum,
TS28: thalamus	Strong	Regionally restricted		Strong to moderate expression in ventral, medial, and lateral thalamus.
TS28: hypothalamus	Strong	Regionally restricted		Expression is in dorsal nuclei and moderate in ventral nuclei.
TS28: brainstem	Strong	Regionally restricted		Expression is in tegmental pontine nuclei.
TS28: accumbens nucleus	Moderate
TS28: amygdala	Moderate
TS28: arcuate nucleus	Moderate
TS28: area postrema	Moderate
TS28: brainstem reticular formation	Moderate
TS28: cerebellum internal granule cell layer	Moderate
TS28: cochlear nucleus	Moderate
TS28: dorsal thalamus	Moderate
TS28: epithalamus	Moderate
TS28: globus pallidus	Moderate
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb granule cell layer	Moderate
TS28: reticular thalamic nucleus	Moderate
TS28: substantia nigra	Moderate
TS28: trigeminal V spinal sensory nucleus	Moderate
TS28: caudate-putamen	Weak
TS28: inferior olivary nucleus	Weak
TS28: cerebral aqueduct	Weak	Regionally restricted		Expression is in the subcomissural organ.
TS28: cerebellum molecular layer	Trace
TS28: fimbria hippocampus	Trace
TS28: paraventricular hypothalamic nucleus	Trace