Gene Expression Data
Assay Details
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Assay
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Reference:
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J:25500
Puschel AW, et al., Murine semaphorin D/collapsin is a member of a diverse gene family and creates domains inhibitory for axonal extension. Neuron. 1995 May;14(5):941-8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4360718
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Gene symbol:
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Sema3a
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Gene name:
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sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
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Probe:
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SemD cDNA3
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Probe preparation:
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labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
4A:
embryonic day 11.5
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: vertebral pre-cartilage condensation |
Present |
Regionally restricted |
4A
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Expression was detected in the ventral half of the prevertebrae.
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| TS19: trunk mesenchyme |
Present |
Regionally restricted |
4A
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Expression was detected in lateral plate mesoderm and in the mesenchyme surrounding dorsal root ganglia and covering the dorsal part of the spinal cord.
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| TS19: lung |
Present |
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4A
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| TS19: future spinal cord |
Weak |
Regionally restricted |
4A
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Expression was weak in the motor column.
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Specimen
4C:
embryonic day 12.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: trunk mesenchyme |
Present |
Regionally restricted |
4C
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Expression was detected in mesodermal tissue surrounding dorsal root ganglia and spinal cord.
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| TS20: spinal cord |
Present |
Regionally restricted |
4C
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Expression was detected in the ventral half of the spinal cord.
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Specimen
4E:
embryonic day 15.5
(more )
Specimen
4E:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: trunk mesenchyme |
Present |
Regionally restricted |
4E
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Mesodermal expression was largely restricted to cells around the dorsal aorta.
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| TS23: spinal cord |
Present |
Regionally restricted |
4E
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Expression was detected in the ventral two-thirds of the thoracic spinal cord.
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Analysis Tools