Gene Expression Data
Assay Details
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Assay
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Reference:
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J:18760
Yamazaki M, et al., C-kit gene is expressed by skin mast cells in embryos but not in puppies of Wsh/Wsh mice: age-dependent abolishment of c-kit gene expression. Blood. 1994 Jun 15;83(12):3509-16
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4360065
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Gene symbol:
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Kit
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Gene name:
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Kit proto-oncogene receptor tyrosine kinase
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Probe:
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cKit cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4A:
postnatal day 5
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: skin |
Present |
Single cells |
4A
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Some mast cells showed expression.
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Specimen
4D:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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KitW-sh/KitW-sh
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: skin |
Trace |
Single cells |
4D
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Mast cells rarely showed expression in the mutant.
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Specimen
Table 1:
embryonic day 17.5
(more )
Specimen
Table 1:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: skin |
Present |
Regionally restricted |
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Expression was detected in 232 +/-27 cells per cm of skin section.
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Specimen
Table 1:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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KitW-sh/KitW-sh
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: skin |
Present |
Regionally restricted |
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Expression was detected in 9 +/-1 cells per cm of skin section.
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Specimen
Table 1:
postnatal newborn
(more )
Specimen
Table 1:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: skin |
Present |
Regionally restricted |
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Expression was detected in 220 +/-16 cells per cm of skin section.
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Specimen
Table 1:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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KitW-sh/KitW-sh
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: skin |
Present |
Regionally restricted |
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Expression was detected in 7 +/-1 cells per cm of skin section.
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Specimen
Table 1:
postnatal day 5
(more )
Specimen
Table 1:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: skin |
Present |
Regionally restricted |
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Expression was detected in 165 +/-8 cells per cm of skin section.
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Specimen
Table 1:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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KitW-sh/KitW-sh
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: skin |
Present |
Regionally restricted |
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Expression was detected in 1 +/-1 cells per cm of skin section.
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Analysis Tools