Gene Expression Data
Assay Details
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Assay
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Reference:
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J:15850
Guillemot F, et al., Mammalian achaete-scute homolog 1 is required for the early development of olfactory and autonomic neurons. Cell. 1993 Nov 5;75(3):463-76
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4359439
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Gene symbol:
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Tubb3
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Gene name:
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tubulin, beta 3 class III
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Results
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Specimen
wild type:
postnatal newborn
(more  )
Specimen
wild type:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: olfactory epithelium |
Present |
Regionally restricted |
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Expression is present in the neurons but not the basal and sustentacular cells.
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Specimen
mutant:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: olfactory epithelium |
Present |
Regionally restricted |
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The olfactory epithelium is essentially devoid of expression, with the exception of a few areas where scattered immunoreactive cells could be observed.
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Specimen
3C:
embryonic day 13.5
(more )
Note: This is a horizontal section.
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is a horizontal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: olfactory epithelium |
Present |
Regionally restricted |
3C
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Expression is present in the neurons, which are a large proportion of the cells in the olfactory epithelium at this age.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is a horizontal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: olfactory epithelium |
Present |
Scattered |
3D
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Only scattered immunoreactive cells are seen. They are grouped in the caudal region of the nasal cavities, in larger numbers along the nasal septum than in the turbinates. Large portions of the epithelium show no staining.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is a horizontal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: olfactory epithelium |
Present |
Scattered |
3E
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Only scattered immunoreactive cells are seen. They are grouped in the caudal region of the nasal cavities, in larger numbers along the nasal septum than in the turbinates. Large portions of the epithelium show no staining.
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Specimen
wild type:
embryonic day 11.5-12.5
(more )
Specimen
wild type:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5-12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
mutant:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 11.5-12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: olfactory epithelium |
Present |
Regionally restricted |
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Expression is present in neurons. Expression is consistently lower in the mutant compared to wild type.
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Analysis Tools