Probe:	Tbx3 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	1F	Expression at the posterior margin of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	1G	Expression in the anterior and posterior proximal mesenchyme of both forelimb and hindlimb buds.
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	1G	Expression in the anterior and posterior proximal mesenchyme of both forelimb and hindlimb buds.
TS17: forelimb bud apical ectodermal ridge	Present		1G
TS17: hindlimb bud apical ectodermal ridge	Present		1G

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	1H	Expanded anterior and posterior expression domains. Expression in anterior mesenchyme restricted to proximal half of limb margin. In posterior domain, expression extends from point of limb articulation with body wall to middle of autopod .
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	1H	Expanded anterior and posterior expression domains. Expression in anterior mesenchyme restricted to proximal half of limb margin. In posterior domain, expression extends from point of limb articulation with body wall to middle of autopod .

Structure	Level	Pattern	Image	Note
TS20: foot	Present	Regionally restricted	1I	Expression at the anterior and posterior margins exhibits a symmetrical proximodistal boundary.
TS20: hand	Present	Regionally restricted	1I	Expression at the anterior and posterior margins exhibits a symmetrical proximodistal boundary.
TS20: foot mesenchyme	Present	Regionally restricted	1I	Expression only in the mesenchyme surrounding digits I and V.
TS20: hand mesenchyme	Present	Regionally restricted	1I	Expression only in the mesenchyme surrounding digits I and V.
TS20: interdigital region between forelimb digits 1 and 2 mesenchyme	Present		1I	Expression only in the mesenchyme surrounding digits I and V.
TS20: interdigital region between forelimb digits 4 and 5 mesenchyme	Present		1I	Expression only in the mesenchyme surrounding digits I and V.
TS20: interdigital region between hindlimb digits 1 and 2	Present		1I	Expression only in the mesenchyme surrounding digits I and V.
TS20: interdigital region between hindlimb digits 4 and 5	Present		1I	Expression only in the mesenchyme surrounding digits I and V.
TS20: foot epithelium	Present	Regionally restricted	1I	Expression in the AER.
TS20: hand epithelium	Present	Regionally restricted	1I	Expression in the AER.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	1J	Persistent expression in the mesenchyme underlying the ectoderm at the tips of all of the digits.
TS21: hand mesenchyme	Present	Regionally restricted	1J	Persistent expression in the mesenchyme underlying the ectoderm at the tips of all of the digits.
TS21: forelimb interdigital region	Absent		1J
TS21: hindlimb interdigital region	Absent		1J

Structure	Level	Pattern	Image	Note
TS19: footplate apical ectodermal ridge	Present		2C	Expression through the entire length of the distal AER.
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	2C	Expression in posterior and anterior mesenchyme.