Probe:	Olig2 probe4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted		Expression in the ventral spinal cord

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	2A	Expression declines in intensity and becomes progressively restricted to a very narrow band within the ventral neuroepithelium of the spinal cord.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	2E	Increased expression in the ventral neuroepithelium of the spinal cord.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	2I	Individual expressing cells detected in the gray matter.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	2M	Expressing cells have begun to disperse through gray matter, in a ventral-to-dorsal manner similar to that of Pdgfra-expressing cells.
TS22: spinal cord ventricular layer	Present	Regionally restricted	2M	Some expressing cells remain in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted	2Q	Expression in scattered cells throughout the spinal cord.
TS23: spinal cord ventricular layer	Absent		2Q	No expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted	3A	Expression domain in the ventricular zone overlaps with Olig2.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted	3D	Expression domain in the ventricular zone overlaps with Olig2.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	3G	Expression in cells migrating through the gray matter.

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata ventricular layer	Present	Regionally restricted	5B	Foci of expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: pons ventricular layer	Present	Regionally restricted	5D	Foci of expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: thalamus ventricular layer	Present	Regionally restricted	5F	Foci of expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata ventricular layer	Present		5H	Expression in the ventricular zone.
TS22: medulla oblongata	Present	Regionally restricted	5H	Expressing cells have begun to disperse through the gray matter.

Structure	Level	Pattern	Image	Note
TS22: pons ventricular layer	Present		5J	Expression in the ventricular zone.
TS22: pons	Present	Regionally restricted	5J	Expressing cells have begun to disperse through the gray matter.

Structure	Level	Pattern	Image	Note
TS22: thalamus ventricular layer	Present		5L	Expression in the ventricular zone.
TS22: thalamus	Present	Regionally restricted	5L	Expressing cells have begun to disperse through the gray matter.