Probe:	p4-is
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		1B , 1A	Expression in the single layer of cells facing the blastocoel (primary endoderm).

Structure	Level	Pattern	Image	Note
TS7: extraembryonic visceral endoderm	Present		1D , 1C	Expression in all visceral endoderm cells, forming a low columnar epithelium surrounding the egg cylinder and extending up to the ectoplacental cone.
TS7: primitive endoderm	Present		1D , 1C	Expression in all visceral endoderm cells, forming a low columnar epithelium surrounding the egg cylinder and extending up to the ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS9: extraembryonic visceral endoderm	Present		1F , 1E	Expression in the columnar visceral endoderm.
TS9: primitive endoderm	Absent		1F , 1E	No expression in squamous endoderm cells surrounging the lower embryonic region.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic visceral endoderm	Present	Regionally restricted	1H , 1G	Expression restricted to columnar visceral endoderm, often appearing to be more abundant on the posterior side.
TS11: embryo	Absent		1G , 1H
TS11: extraembryonic ectoderm	Absent		1G , 1H
TS11: extraembryonic mesoderm	Absent		1G , 1H

Structure	Level	Pattern	Image	Note
TS12: extraembryonic visceral endoderm	Present		2A , 2B	Expression in yolk sac endoderm.
TS12: yolk sac endoderm	Present		2A , 2B	Expression in yolk sac endoderm.
TS12: embryo endoderm	Absent		2A , 2B	Border of expression corresponds approximately with the border between extraembryonic and embryonic endoderm.
TS12: foregut diverticulum endoderm	Absent		2A , 2B	No expression in foregut endoderm.

Structure	Level	Pattern	Image	Note
TS12: extraembryonic visceral endoderm	Present		2D , 2C	Expression in yolk sac endoderm.
TS12: yolk sac endoderm	Present		2D , 2C	Expression in yolk sac endoderm.
TS12: embryo endoderm	Absent		2D , 2C	Border of expression corresponds approximately with the border between extraembryonic and embryonic endoderm.
TS12: foregut diverticulum endoderm	Absent		2C , 2D	No expression in foregut endoderm.

Structure	Level	Pattern	Image	Note
TS12: extraembryonic visceral endoderm	Present		2E , 2F	Expression in yolk sac endoderm.
TS12: yolk sac endoderm	Present		2E , 2F	Expression in yolk sac endoderm.
TS12: embryo endoderm	Absent		2E , 2F	Border of expression corresponds approximately with the border between extraembryonic and embryonic endoderm.
TS12: foregut diverticulum endoderm	Absent		2E , 2F	No expression in foregut endoderm.

Structure	Level	Pattern	Image	Note
TS12: extraembryonic visceral endoderm	Present		2G , 2H	Expression in yolk sac endoderm.
TS12: embryo endoderm	Absent		2G , 2H	Border of expression corresponds approximately with the border between extraembryonic and embryonic endoderm.
TS12: foregut diverticulum endoderm	Absent		2H , 2G	No expression in foregut endoderm.

Structure	Level	Pattern	Image	Note
TS13: yolk sac endoderm	Present		3B , 3A
TS13: embryo endoderm	Ambiguous		3A , 3B	Translational region of cells expressing at a low level between the dorsal embryonic endoderm and yolk sac endoderm.
TS13: foregut-midgut junction endoderm	Strong	Regionally restricted	3A , 3B	Strong expression in liver cells growing ventrally from the ventral wall of the foregut-midgut junction.

Structure	Level	Pattern	Image	Note
TS13: yolk sac endoderm	Present		3C , 3D
TS13: embryo endoderm	Ambiguous		3C , 3D	Translational region of cells expressing at a low level between the dorsal embryonic endoderm and yolk sac endoderm.
TS13: foregut-midgut junction endoderm	Strong		3C , 3D	Strong expression in liver cells growing ventrally from the ventral wall of the foregut-midgut junction.

Structure	Level	Pattern	Image	Note
TS15: yolk sac endoderm	Present		3F , 3E
TS15: hepatic primordium	Strong		3F , 3E
TS15: midgut	Moderate		3E , 3F	Moderate expression in the midgut, including the primordium of the pancreas.
TS15: pancreas primordium	Moderate		3E , 3F	Moderate expression in the midgut, including the primordium of the pancreas.
TS15: gallbladder primordium	Weak		3E , 3F

Structure	Level	Pattern	Image	Note
TS15: yolk sac endoderm	Present		3H , 3G
TS15: hepatic primordium	Strong		3G , 3H
TS15: midgut	Moderate		3H , 3G	Moderate expression in the midgut, including the primordium of the pancreas.
TS15: pancreas primordium	Moderate		3H , 3G	Moderate expression in the midgut, including the primordium of the pancreas.

Structure	Level	Pattern	Image	Note
TS15: hindgut	Weak

Structure	Level	Pattern	Image	Note
TS17: liver	Present		4B , 4A
TS17: mesonephric tubule	Present		4B , 4A
TS17: stomach	Present	Regionally restricted	4B , 4A	Expression in the stomach in a gradient increasing toward the caudal and ventral region.

Structure	Level	Pattern	Image	Note
TS17: liver	Present		4C , 4D
TS17: stomach	Present	Regionally restricted	4D , 4C	Expression in the stomach in a gradient increasing toward the caudal and ventral region.

Structure	Level	Pattern	Image	Note
TS17: liver	Present	Regionally restricted	4F , 4E	Expression apparently restricted to hepatocytes.

Structure	Level	Pattern	Image	Note
TS19: liver	Present		5B , 5A	Authors report that the expression pattern is similar to the previous stage.
TS19: mesonephric tubule	Present		5B , 5A	Authors report that the expression pattern is similar to the previous stage.
TS19: stomach	Present		5B , 5A	Authors report that the expression pattern is similar to the previous stage.

Structure	Level	Pattern	Image	Note
TS21: liver	Present		5D , 5C
TS21: pancreas	Present		5D , 5C
TS21: stomach	Present		5D , 5C
TS21: renal tubule	Strong		5D , 5C

Structure	Level	Pattern	Image	Note
TS21: gut	Present		5F , 5E
TS21: liver	Present		5F , 5E
TS21: pancreas	Present		5F , 5E
TS21: rectum	Present		5F , 5E
TS21: hindgut	Present	Regionally restricted	5F , 5E	Moderate to high expression extends from the midgut caudally through the hindgut, ending apparently within the endoderm approximately at the junction between the rectum and the anus.
TS21: midgut	Present	Regionally restricted	5F , 5E	Moderate to high expression extends from the midgut caudally through the hindgut, ending apparently within the endoderm approximately at the junction between the rectum and the anus.

Structure	Level	Pattern	Image	Note
TS21: rectum	Present		5H , 5G	Expression apparently ends within the endoderm approximately at the junction between the rectum and the anus.
TS21: anal region	Absent		5H , 5G	Expression apparently ends within the endoderm approximately at the junction between the rectum and the anus.