Gene Expression Data
Assay Details
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Assay
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Reference:
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J:78363
Takeda H, et al., Positional cloning of the gene LIMBIN responsible for bovine chondrodysplastic dwarfism. Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10549-54
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3846182
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Gene symbol:
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Evc2
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Gene name:
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EvC ciliary complex subunit 2
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Probe:
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Lbn cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
5A:
embryonic day 17.0
(more  )
Note: Specimen was decalcified in 10% (wt/vol) EDTA for 1 day.
Specimen
5A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen was decalcified in 10% (wt/vol) EDTA for 1 day.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: tibia |
Strong |
Regionally restricted |
5A
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Expression is strong in proliferating chondrocytes and is weak in other resting and hypertrophic chondrocytes in the epiphysial growth plates.
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Specimen
5C:
embryonic day 17.0
(more )
Note: Specimen was decalcified in 10% (wt/vol) EDTA for 1 day.
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen was decalcified in 10% (wt/vol) EDTA for 1 day.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: tibia |
Strong |
Regionally restricted |
5C
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Expression is strong in proliferating chondrocytes and is weak in other resting and hypertrophic chondrocytes in the epiphysial growth plates.
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Specimen
5E:
postnatal day 35
(more )
Note: Specimen was decalcified in 10% (wt/vol) EDTA for 7 days.
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 35
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen was decalcified in 10% (wt/vol) EDTA for 7 days.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Strong |
Regionally restricted |
5E
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Expression is strong in proliferating chondrocytes and is absent in resting and hypertrophic chondrocytes.
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Specimen
5G:
postnatal day 35
(more )
Note: Specimen was decalcified in 10% (wt/vol) EDTA for 7 days.
Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 35
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen was decalcified in 10% (wt/vol) EDTA for 7 days.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Strong |
Regionally restricted |
5G
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Expression is strong in proliferating chondrocytes and is absent in resting and hypertrophic chondrocytes.
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Specimen
5I:
postnatal day 35
(more )
Note: Specimen was decalcified in 10% (wt/vol) EDTA for 7 days.
Specimen
5I:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 35
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Specimen was decalcified in 10% (wt/vol) EDTA for 7 days.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Strong |
Regionally restricted |
5I
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In the metaphysis expression is strong in osteoblasts and osteoclasts on the bone surface and is weak in osteocytes.
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Analysis Tools