Probe:	Dlx-2 cDNA1
Probe preparation:	labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: medial ganglionic eminence	Present		1e
TS20: cerebral cortex	Absent		1e	No expression in the entire neuroepithelium of the cerebral cortex
TS20: diencephalon	Present	Regionally restricted	1e	Second expression domain in the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT). No expression in the mammillary area (MA).
TS20: hypothalamus	Present	Regionally restricted	1e	Second expression domain in the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT). No expression in the mammillary area (MA).
TS20: thalamus	Present	Regionally restricted	1e	Second expression domain in the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT). No expression in the mammillary area (MA).

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1f	Expression domain includes the septum (SE).

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	1g	No expression in the mammillary area (MA).
TS20: medial ganglionic eminence	Present		1g

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1h	Expression domain includes the medial ganglionic eminence (MGE). No expression in the supraoptic/paraventricular area (SPV), the eminentia thalami (EMT) and posterior preoptic area (POP).
TS20: diencephalon	Present	Regionally restricted	1h	Second expression domain in the suprachiasmic area (SCH), posterior entopeduncular area (PEP) and ventral thalamus (VT). Weak expression in lateral parts of the tuberal hypothalamus (TU).
TS20: thalamus	Present	Regionally restricted	1h	Second expression domain in the suprachiasmic area (SCH), posterior entopeduncular area (PEP) and ventral thalamus (VT). Weak expression in lateral parts of the tuberal hypothalamus (TU).
TS20: medial ganglionic eminence	Present		1h

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	2d	Second expression domain in the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT).
TS20: hypothalamus	Present	Regionally restricted	2d	Second expression domain in the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT).
TS20: thalamus	Present	Regionally restricted	2d	Second expression domain in the hypothalamic cell cord (HCC), posterior entopeduncular area (PEP) and ventral thalamus (VT).
TS20: lateral ganglionic eminence	Present		2d

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	2e	Second expression domain in the hypothalamic cell cord (HCC). No expression in the supraoptic/paraventricular area (SPV) and the eminentia thalami (EMT). No expression in the median part of the TU.
TS20: hypothalamus	Present	Regionally restricted	2e	Second expression domain in the hypothalamic cell cord (HCC). No expression in the supraoptic/paraventricular area (SPV) and the eminentia thalami (EMT). No expression in the median part of the TU.
TS20: lateral ganglionic eminence	Present		2e
TS20: medial ganglionic eminence	Present		2e

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2f	Expression domain includes the medial ganglionic eminence (MGE), lateral ganglionic eminence LGE), and the septum (SE). No expression in the posterior preoptic area (POP).
TS20: diencephalon	Present	Regionally restricted	2f	Second expression domain in the suprachiasmic area (SCH).
TS20: lateral ganglionic eminence	Present		2f
TS20: medial ganglionic eminence	Present		2f

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	5Hd	Expression domain includes the septum (SE).
TS20: lateral ganglionic eminence	Present		5Hd

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	5Id	Expression domain includes the septum (SE).
TS20: lateral ganglionic eminence	Present		5Id
TS20: medial ganglionic eminence	Present		5Id

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	5Jd	No expression in the supraoptic/paraventricular area (SPV) or eminentia thalami (EMT). Dlx-negative region seen as a gap that tapers into the optic stalk paramedian area.
TS20: oral region	Present	Regionally restricted	5Jd	Expression in the teeth anlage.
TS20: retina	Present		5Jd
TS20: caudal ganglionic eminence	Absent		5Jd

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present		5Kd