Gene Expression Data
Assay Details
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Assay
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Reference:
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J:138310
Xu C, et al., Expression of Robo/Slit and Semaphorin/Plexin/Neuropilin family members in the developing hypothalamic paraventricular and supraoptic nuclei. Gene Expr Patterns. 2008 Sep;8(7-8):502-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3845299
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Gene symbol:
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Slit1
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Gene name:
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slit guidance ligand 1
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Probe:
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Slit1 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Fixative was Methancarn (60% methanol,
30% chloroform, and 10% acetic acid).
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Results
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Specimen
2D1:
embryonic day 12.5
(more  )
Specimen
2D1:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Strong |
Regionally restricted |
2_D1
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Expression is non-uniformly at the fringes of the medial, dorsal, and ventral borders of the paraventricular nucleus/supraoptic nucleus progenitor.
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Specimen
2D2:
embryonic day 13.5
(more )
Specimen
2D2:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus |
Present |
Regionally restricted |
2_D2
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Expression is at the borders above and below the paraventricular nucleus/supraoptic nucleus progenitor region.
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Specimen
2D3:
embryonic day 14.5
(more )
Specimen
2D3:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Present |
Regionally restricted |
2_D3
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Expression is at the borders above and below the paraventricular nucleus/supraoptic nucleus progenitor region.
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Specimen
2D4, D5:
embryonic day 15.5
(more )
Note: (D5) is a higher magnification of the boxed area in (D4).
Specimen
2D4, D5:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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(D5) is a higher magnification of the boxed area in (D4).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Strong |
Regionally restricted |
2_D5
,
2_D4
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Expression is outside of the paraventricular nucleus dorsal boundary.
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Analysis Tools