Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:138310
Xu C, et al., Expression of Robo/Slit and Semaphorin/Plexin/Neuropilin family members in the developing hypothalamic paraventricular and supraoptic nuclei. Gene Expr Patterns. 2008 Sep;8(7-8):502-7
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3845298
|
|
Gene symbol:
|
Nrp2
|
|
Gene name:
|
neuropilin 2
|
|
Probe:
|
Nrp2 cDNA4
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
Fixative was Methancarn (60% methanol,
30% chloroform, and 10% acetic acid).
|
|
|
Results
|
Specimen
2C1:
embryonic day 12.5
(more  )
Specimen
2C1:
(close  )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus |
Strong |
Regionally restricted |
2_C1
|
Expression is in the paraventricular nucleus/supraoptic nucleus progenitor region.
|
| TS20: thalamus |
Strong |
Regionally restricted |
2_C1
|
Expression is dorsal to the paraventricular nucleus/supraoptic nucleus progenitor region.
|
Specimen
2C2:
embryonic day 13.5
(more )
Specimen
2C2:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus |
Present |
Regionally restricted |
2_C2
|
Expression is in the paraventricular nucleus/supraoptic nucleus progenitor region.
|
Specimen
2C3, C5:
embryonic day 14.5
(more )
Note: (C5) is a higher magnification of the boxed area in (C3).
Specimen
2C3, C5:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
(C5) is a higher magnification of the boxed area in (C3).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hypothalamus |
Present |
Regionally restricted |
2_C3
,
2_C5
|
Expression is in the paraventricular nucleus/supraoptic nucleus progenitor region.
|
Specimen
2C4:
embryonic day 15.5
(more )
Specimen
2C4:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Present |
Regionally restricted |
2_C4
|
Expression is in the mature paraventricular nucleus, and is high in areas above and below the nucleus.
|
|
Analysis Tools