Antibody:	anti-galanin
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to beta-galactosidase

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme derived from neural crest	Strong		1A	Authors report strong expression in undifferentiated cephalic mesenchyme and trunk mesenchyme/somites of both mesodermal and neural crest origin.
TS17: mesenchyme derived from head mesoderm	Strong		1A	Authors report strong expression in undifferentiated cephalic mesenchyme and trunk mesenchyme/somites of both mesodermal and neural crest origin.
TS17: trunk mesenchyme derived from neural crest	Strong		1A	Authors report strong expression in undifferentiated cephalic mesenchyme and trunk mesenchyme/somites of both mesodermal and neural crest origin.
TS17: trunk somite	Strong		1A	Authors report strong expression in undifferentiated cephalic mesenchyme and trunk mesenchyme/somites of both mesodermal and neural crest origin.
TS17: 2nd branchial arch mesenchyme	Present		1A
TS17: endocardial cushion	Strong	Regionally restricted	1A	Strong expression in mesenchymal spiral ridges of outflow tract of heart and endocardial cushions.
TS17: outflow tract	Strong	Regionally restricted	1A	Strong expression in mesenchymal spiral ridges of outflow tract of heart and endocardial cushions.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme derived from neural crest	Strong		1B
TS17: mesenchyme derived from head mesoderm	Strong		1B
TS17: 2nd branchial arch mesenchyme	Present		1B
TS17: endocardial cushion	Strong	Regionally restricted	1B	Strong expression in mesenchymal spiral ridges of outflow tract of heart and endocardial cushions.
TS17: outflow tract	Strong	Regionally restricted	1B	Strong expression in mesenchymal spiral ridges of outflow tract of heart and endocardial cushions.

Structure	Level	Pattern	Image	Note
TS21: cartilage	Strong		1C	Strongest expression in the cephalic/craniofacial mesenchyme, and in proliferating chondrocytes in bones of both neural crest and mesoderm origin.
TS21: head mesenchyme	Strong		1C	Strongest expression in the cephalic/craniofacial mesenchyme, and in proliferating chondrocytes in bones of both neural crest and mesoderm origin.
TS21: upper jaw	Present	Regionally restricted	1C	Expression in the roof of the oropharynx,
TS21: basioccipital pre-cartilage condensation	Present		1C
TS21: basisphenoid pre-cartilage condensation	Present		1C
TS21: lower jaw	Present		1C
TS21: nasal capsule	Present		1C
TS21: nasal septum	Present		1C
TS21: otic capsule	Present		1C
TS21: primary palate	Present		1C
TS21: tongue	Present	Regionally restricted	1C	Expression in the proximal tongue
TS21: dorsal root ganglion	Present		1C
TS21: trigeminal ganglion	Present		1C
TS21: pleura	Present		1C
TS21: midgut	Strong	Regionally restricted	1C	Expression in the layer of the midgut between the epithelia and the mesonephros.

Structure	Level	Pattern	Image	Note
TS21: cartilage	Strong		1D	Strongest expression in the cephalic/craniofacial mesenchyme, and in proliferating chondrocytes in bones of both neural crest and mesoderm origin.
TS21: head mesenchyme	Strong		1D	Strongest expression in the cephalic/craniofacial mesenchyme, and in proliferating chondrocytes in bones of both neural crest and mesoderm origin.
TS21: upper jaw	Present	Regionally restricted	1D	Expression in the roof of the oropharynx,
TS21: basioccipital pre-cartilage condensation	Present		1D
TS21: basisphenoid pre-cartilage condensation	Present		1D
TS21: lower jaw	Present		1D
TS21: nasal capsule	Present		1D
TS21: nasal septum	Present		1D
TS21: otic capsule	Present		1D
TS21: primary palate	Present		1D
TS21: tongue	Present	Regionally restricted	1D	Expression in the proximal tongue
TS21: dorsal root ganglion	Present		1D
TS21: trigeminal ganglion	Present		1D

Structure	Level	Pattern	Image	Note
TS23: skin	Present		1E
TS23: rib	Present		1E	Authors state that expression in the bones, head, gut and heart was very similar to that seen at E13

Structure	Level	Pattern	Image	Note
TS23: skin	Present		1F
TS23: femur	Present		1F	Authors state that expression in the bones, head, gut and heart was very similar to that seen at E13

Structure	Level	Pattern	Image	Note
TS18: head mesenchyme	Strong		2A	Strong expression in cranial and trunk mesenchyme, including tail bud mesenchyme, the condensing mesoderm of the somites, the craniofacial mesenchyme and the developing bone and cartilage.
TS18: tail mesenchyme	Strong		2A	Strong expression in cranial and trunk mesenchyme, including tail bud mesenchyme, the condensing mesoderm of the somites, the craniofacial mesenchyme and the developing bone and cartilage.
TS18: trunk mesenchyme	Strong		2A	Strong expression in cranial and trunk mesenchyme, including tail bud mesenchyme, the condensing mesoderm of the somites, the craniofacial mesenchyme and the developing bone and cartilage.
TS18: trunk sclerotome	Present		2A	Expression in the dense and diffuse portions of the mesenchymal sclerotomes, which are precursors of the vertebra.
TS18: vertebral pre-cartilage condensation	Present		2A	Expression in the dense and diffuse portions of the mesenchymal sclerotomes, which are precursors of the vertebra.

Structure	Level	Pattern	Image	Note
TS19: vertebral pre-cartilage condensation	Present		2B	Expression in the dense and diffuse portions of the mesenchymal sclerotomes, which are precursors of the vertebra.

Structure	Level	Pattern	Image	Note
TS20: vertebral pre-cartilage condensation	Present		2C	Expression in the dense and diffuse portions of the mesenchymal sclerotomes, which are precursors of the vertebra.

Structure	Level	Pattern	Image	Note
TS21: vertebral pre-cartilage condensation	Present		2D	Expression in the dense and diffuse portions of the mesenchymal sclerotomes, which are precursors of the vertebra.

Structure	Level	Pattern	Image	Note
TS22: cartilage	Strong		3A	Strongest expression in the craniofacial mesenchyme, and in proliferating chondrocytes in bones of both neural crest and mesoderm origin.
TS22: head mesenchyme	Strong		3A	Strongest expression in the craniofacial mesenchyme, and in proliferating chondrocytes in bones of both neural crest and mesoderm origin.
TS22: upper jaw	Present	Regionally restricted	3A	Expression in the roof of the oropharynx
TS22: basioccipital cartilage condensation	Present		3A
TS22: basisphenoid cartilage condensation	Present		3A
TS22: lower jaw	Present		3A
TS22: nasal capsule	Present		3A
TS22: nasal septum	Present		3A
TS22: otic capsule	Present		3A
TS22: primary palate	Present		3A

Structure	Level	Pattern	Image	Note
TS22: cartilage	Strong		3B	Strongest expression in the craniofacial mesenchyme, and in proliferating chondrocytes in bones of both neural crest and mesoderm origin.
TS22: head mesenchyme	Strong		3B	Strongest expression in the craniofacial mesenchyme, and in proliferating chondrocytes in bones of both neural crest and mesoderm origin.
TS22: upper jaw	Present	Regionally restricted	3B	Expression in the roof of the oropharynx
TS22: basioccipital cartilage condensation	Present		3B
TS22: basisphenoid cartilage condensation	Present		3B
TS22: lower jaw	Present		3B
TS22: nasal capsule	Present		3B
TS22: nasal septum	Present		3B
TS22: otic capsule	Present		3B
TS22: primary palate	Present		3B
TS22: tongue	Present		3B	Expression in the proximal tongue.

Structure	Level	Pattern	Image	Note
TS21: atrioventricular endocardial cushion	Present		4A
TS21: aortico-pulmonary spiral septum	Present	Regionally restricted	4A	Expression in endocardial cushions and spiral septum in small cells with large intercellular spaces (i.e. similar to mesenchymal cells)
TS21: endocardial cushion	Present	Regionally restricted	4A	Expression in endocardial cushions and spiral septum in small cells with large intercellular spaces (i.e. similar to mesenchymal cells)
TS21: heart atrium	Present		4A	Authors state that expression in the atrium and ventricle is less defined.
TS21: heart ventricle	Present		4A	Authors state that expression in the atrium and ventricle is less defined.

Structure	Level	Pattern	Image	Note
TS21: cartilage	Strong		4B	Strongest expression in the cephalic/craniofacial mesenchyme, and in proliferating chondrocytes in bones of both neural crest and mesoderm origin.
TS21: head mesenchyme	Strong		4B	Strongest expression in the cephalic/craniofacial mesenchyme, and in proliferating chondrocytes in bones of both neural crest and mesoderm origin.