Gene Expression Data
Assay Details
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Assay
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Reference:
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J:147752
Gromley A, et al., Transient expression of the Arf tumor suppressor during male germ cell and eye development in Arf-Cre reporter mice. Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6285-90
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3843625
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Gene symbol:
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Cdkn2a
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Gene name:
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cyclin dependent kinase inhibitor 2A
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Antibody:
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anti-Cdkn2a
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Assay notes:
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Primary antibody treatment was followed by Tyramide Signal Amplification (Invitrogen) using the manufacturer-supplied protocol.
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Results
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Specimen
1A:
postnatal day 10
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 10
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatogonium |
Present |
Regionally restricted |
1A
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Fluorescence is observed in spermatogonia rimming the seminiferous tubules, but not in intraluminal cells.
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Specimen
1B:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Cdkn2atm4Cjs/Cdkn2atm4Cjs
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Age:
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postnatal day 21
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatogonium |
Strong |
Uniform |
1B
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Expression is uniform in spermatogonia.
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Specimen
not shown:
postnatal day 21
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 21
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1C:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Cdkn2atm3(cre)Cjs/Cdkn2atm4Cjs
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Age:
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postnatal day 21
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatogonium |
Present |
Spotted |
1C
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Fluorescence persists in only some cells in the seminiferous tubules.
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Analysis Tools
