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Gene Expression Data
Assay Details
Assay
Reference: J:78343 Loebel DA, et al., Isolation of differentially expressed genes from wild-type and Twist mutant mouse limb buds. Genesis. 2002 Jul;33(3):103-13
Assay type: RT-PCR
MGI Accession ID: MGI:3842731
Gene symbol: Alx4
Gene name: aristaless-like homeobox 4
Results
Image: 1a Alx4 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 360 bp Amount Genetic BackgroundMutant Allele(s)Sex
H2O Control
Wild-type forelimb 1 E10.5 (a) TS17: forelimb bud Present Not Specified; total RNA Not Specified Not Specified
Wild-type forelimb 1:10 E10.5 (a) TS17: forelimb bud Present Not Specified; total RNA Not Specified Not Specified
Wild-type forelimb 1:100 E10.5 (a) TS17: forelimb bud Not Specified Not Specified; total RNA Not Specified Not Specified
Mutant forelimb 1 E10.5 (a) TS17: forelimb bud Present (b) Not Specified; total RNA involves: 129S7/SvEvBrd * C57BL/6 Twist1tm1Bhr/Twist1tm1BhrNot Specified
Mutant forelimb 1:10 E10.5 (a) TS17: forelimb bud Not Specified Not Specified; total RNA involves: 129S7/SvEvBrd * C57BL/6 Twist1tm1Bhr/Twist1tm1BhrNot Specified
Mutant forelimb 1:100 E10.5 (a) TS17: forelimb bud Not Specified Not Specified; total RNA involves: 129S7/SvEvBrd * C57BL/6 Twist1tm1Bhr/Twist1tm1BhrNot Specified
No RT Wt Control
No RT Mt Control
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) The analysis showed less amplification from the mutant compared to wild type. The mutant was approximately equivalent to that achieved from amplifying a tenfold dilution of wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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