Probe:	Prox1 homeobox probe
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Weak			This is the first expression seen in the central nervous system.
TS17: optic cup	Weak			There is weak expression in the optic vesicles.

Structure	Level	Pattern	Image	Note
TS17: heart	Present
TS17: pancreas primordium	Present			Expression is present in the enlarged endodermal portion of the dorsal pancreas.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted	3A/B	Expression is found all along the antero-posterior axis of the developing neural tube.
TS20: alar columns	Present	Regionally restricted	3A/B	Expression is found all along the antero-posterior axis of the developing neural tube, with a restriction to the dorsal alar plate.

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	3C/D	Expression is present in postmitotic subventricular neurons in the subventricular region of the tube. There maybe a few labeled cells present in the ventricular region as well.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present		4A/B	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: ganglionic eminence	Present		4A/B	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: medulla oblongata	Present		4A/B	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: telencephalon	Present		4A/B	Areas of expression include the hippocampus.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present		4C/D	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: ganglionic eminence	Present		4C/D	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: medulla oblongata	Present		4C/D	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: midbrain	Weak	Regionally restricted	4C/D	There is weak expression in the roof.
TS20: diencephalon	Present	Regionally restricted	4C/D	Expression is present in the mammillary area.
TS20: thalamus	Present	Regionally restricted	4C/D	Expression levels are strong in the ventral thalmus and moderate in the dorsal thalamus.
TS20: epithalamus	Present	Non-Uniform	4C/D	There is moderate expression in the habenula and faint expression in the epithalmic region.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present		4E/F	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: ganglionic eminence	Present		4E/F	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: medulla oblongata	Present		4E/F	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: pons	Present		4E/F	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: telencephalon	Present		4E/F	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: pancreas	Present		4E/F
TS20: cerebellum primordium	Absent		4E/F
TS20: epithalamus	Present	Non-Uniform	4E/F	There is moderate expression in the habenula and faint expression in the epithalmic region.
TS20: thalamus	Present	Non-Uniform	4E/F	Expression levels are strong in the ventral thalmus and moderate in the dorsal thalamus.
TS20: diencephalon	Present		4E/F	Areas of expression include the mammillary area.
TS20: optic chiasma	Present		4E/F

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present		4G/H	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: ganglionic eminence	Present		4G/H	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: medulla oblongata	Present		4G/H	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: pons	Present		4G/H	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: telencephalon	Present		4G/H	Expression is mainly in the subventricular region containing undifferentiated neurons.
TS20: midbrain	Present		4G/H	Areas of expression include the tegmentum.
TS20: cerebellum primordium	Absent		4G/H
TS20: thalamus	Present	Non-Uniform	4G/H	Expression levels are strong in the ventral thalmus and moderate in the dorsal thalamus.
TS20: diencephalon	Present		4G/H	Areas of expression include the mammillary area.
TS20: hypothalamus	Present	Regionally restricted	4G/H	Areas of expression include the postoptic area.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present	Regionally restricted	5A/B	Expression is restricted to the subventricular region containing undifferentiated neurons.
TS20: hypothalamus	Present	Regionally restricted	5A/B	Expression is restricted to the subventricular region containing undifferentiated neurons.
TS20: medulla oblongata	Present	Regionally restricted	5A/B	Expression is restricted to the subventricular region containing undifferentiated neurons.
TS20: hypothalamus ventricular layer	Absent		5A/B
TS20: medulla oblongata ventricular layer	Absent		5A/B

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present	Regionally restricted	5C/D	Expression is restricted to the subventricular region containing undifferentiated neurons.
TS20: hypothalamus	Present	Regionally restricted	5C/D	Expression is restricted to the subventricular region containing undifferentiated neurons.
TS20: hypothalamus ventricular layer	Absent		5C/D

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Present	Regionally restricted	5E/F	Expression is restricted to the subventricular region containing undifferentiated neurons.
TS20: medulla oblongata ventricular layer	Absent		5E/F
TS20: ganglionic eminence	Present	Regionally restricted	5E/F , 5G/H	Expression is restricted to the subventricular region containing undifferentiated neurons.
TS20: optic chiasma	Present	Regionally restricted	5G/H , 5E/F	Expression is present in the subventricular region of the optic chiasm.

Structure	Level	Pattern	Image	Note
TS26: central nervous system	Trace

Structure	Level	Pattern	Image	Note
TS22: brain	Present		6A/B	Expression levels are decreasing.
TS22: spinal cord	Present		6A/B	Expression levels are decreasing.
TS22: hippocampus	Present		6A/B
TS22: thalamus	Present	Non-Uniform	6A/B	The strongest expression in the brain at this time is found in the dorsal thalamus; expression in the ventral thalamus is much weaker.
TS22: telencephalon	Present	Regionally restricted	6A/B	Expression is present at the base of the telencephalon.
TS22: heart	Present		6A/B
TS22: liver	Present		6A/B
TS22: intercostal muscle	Present		6A/B
TS22: tongue muscle	Present		6A/B

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Present		7A/B	There is transient expression most developing skeletal muscles, including those in the neck and trunk.
TS22: extrinsic ocular muscle	Present		7A/B
TS22: forelimb musculature	Present		7A/B
TS22: hindlimb musculature	Present		7A/B
TS22: intercostal muscle	Present		7A/B
TS22: heart	Present		7A/B
TS22: liver	Present		7A/B

Structure	Level	Pattern	Image	Note
TS23: skeletal muscle	Present			Expression is detected in most skeletal muscles of the embryo.

Structure	Level	Pattern	Image	Note
TS24: skeletal muscle	Absent

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Strong	Regionally restricted	8A/B	Strong expression is present in the non-dividing region of the lens.
TS20: lens vesicle anterior epithelium	Absent		8A/B	There is no expression in the mitotically active lens epithelium.
TS20: neural retinal epithelium	Absent		8A/B

Structure	Level	Pattern	Image	Note
TS20: pancreatic duct	Present		9A/B	Expression is present in the excretory ducts.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present