Sox9 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:142494 Nakazaki H, et al., Transcriptional regulation by Pax3 and TGFbeta2 signaling: a potential gene regulatory network in neural crest development. Int J Dev Biol. 2009;53(1):69-79
Assay type:	RNA in situ
MGI Accession ID:	MGI:3842389
Gene symbol:	Sox9
Gene name:	SRY (sex determining region Y)-box 9

Probe:	Sox9 probe55
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

Specimen (i): embryonic day 10.0 (more

)

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Present		2C (i)

Specimen (ii): embryonic day 10.0; Tgfb2^tm1Doe/Tgfb2^tm1Doe (more

)

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Weak		2C (ii)	Decreased expression compared to wild type.

Specimen (iii): embryonic day 10.0; Pax3^Sp/Pax3^Sp (more

)

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Present		2C (iii)	Increased expression compared to wild type.

Specimen (iv): embryonic day 10.0; Pax3^Sp/Pax3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Present		2C (iv)	There was a small insignificant increase in expression.

Specimen (v): embryonic day 10.0; Pax3^Sp/Pax3⁺, Tgfb2^tm1Doe/Tgfb2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Weak		2C (v)	Decreased expression compared to wild type.

Specimen (vi): embryonic day 10.0; Pax3^Sp/Pax3⁺, Tgfb2^tm1Doe/Tgfb2^tm1Doe (more

)

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Present		2C (vi)	Expression was similar to that seen in wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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