Antibody:	anti-TGFbeta1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to biotin

Structure	Level	Pattern	Image	Note
TS25: epidermis	Strong		2B
TS25: hair follicle	Strong		2B

Structure	Level	Pattern	Image	Note
TS25: palate epithelium	Strong	Regionally restricted	2D	Intense expression in keratinized epithelium of the palate and tongue.
TS25: tongue epithelium	Strong	Regionally restricted	2D	Intense expression in keratinized epithelium of the palate and tongue.

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Weak	Regionally restricted		Weak expression in chondrocytes in the ribs and vertebra.
TS20: vertebral cartilage condensation	Weak	Regionally restricted		Weak expression in chondrocytes in the ribs and vertebra.

Structure	Level	Pattern	Image	Note
TS21: skeleton	Present	Regionally restricted		Expression in chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Strong	Regionally restricted	3A	High levels of expression in the perichondrium surrounding the hypertrophic cartilage in the ribs.

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Strong	Regionally restricted	3B	High levels of expression in the perichondrium; lower levels of expression in hypertrophic cartilage.

Structure	Level	Pattern	Image	Note
TS23: skeleton	Present	Regionally restricted		Expression in chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: skeleton	Strong	Regionally restricted		High levels of expression localized to the periosteum of several bones, as well as in the maturing chondrocytes of various cartilage rudiments.

Structure	Level	Pattern	Image	Note
TS25: chondrocranium	Present	Regionally restricted	3C	Expression in the periosteum and osteogenic zone of endochondral bone.

Structure	Level	Pattern	Image	Note
TS25: chondrocranium	Present	Regionally restricted	3D	Expression in the periosteum of endochondral bone.

Structure	Level	Pattern	Image	Note
TS25: maxilla	Present	Regionally restricted	3F	Expression in osteoblasts.

Structure	Level	Pattern	Image	Note
TS25: skeleton	Strong	Regionally restricted		HIigh levels of expression in osteogenic bone and hypertrophic cartilage.

Structure	Level	Pattern	Image	Note
TS25: enamel organ	Present	Regionally restricted	3G	Expression in the basal region of ameloblasts.

Structure	Level	Pattern	Image	Note
TS20: heart	Present

Structure	Level	Pattern	Image	Note
TS21: heart	Present

Structure	Level	Pattern	Image	Note
TS22: heart	Present

Structure	Level	Pattern	Image	Note
TS23: heart	Present

Structure	Level	Pattern	Image	Note
TS24: heart	Present

Structure	Level	Pattern	Image	Note
TS25: heart	Trace			Expression barely detectable above background.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Absent

Structure	Level	Pattern	Image	Note
TS21: renal tubule	Present	Regionally restricted		Expression in the cuboidal epithelial lining of the metanephric tubules, but not in the basement membranes.

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Present	Regionally restricted		Expression in the cuboidal epithelial lining of the metanephric tubules, but not in the basement membranes.

Structure	Level	Pattern	Image	Note
TS23: renal tubule	Present	Regionally restricted		Expression in the tubule epithelium.

Structure	Level	Pattern	Image	Note
TS25: renal tubule	Weak	Regionally restricted		Decreased expression in the tubular epithelium.
TS25: mesenchyme of metanephros	Present	Regionally restricted		Decreased expression in the tubular epithelium.

Structure	Level	Pattern	Image	Note
TS21: gland	Weak	Regionally restricted		Slight expression in the adrenal gland.

Structure	Level	Pattern	Image	Note
TS23: adrenal cortex	Present	Regionally restricted		Expression in the cortex (presumptive zona fasciculata and zona reticularis).

Structure	Level	Pattern	Image	Note
TS25: adrenal cortex	Strong

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Weak			Authors report slight expression in the intestinal epithelium.

Structure	Level	Pattern	Image	Note
TS23: intestine epithelium	Present	Regionally restricted	6A	Expression in the villi. Highest expression at the villus tip; lowest in the crypts.

Structure	Level	Pattern	Image	Note
TS25: intestine epithelium	Present	Regionally restricted	6B	Expression in the villi, similar to that seen at E15.5.
TS25: intestine	Present	Regionally restricted	6B	Moderate expression in the smooth muscle of the intestine

Structure	Level	Pattern	Image	Note
TS25: esophagus epithelium	Present	Regionally restricted	6C	Expression in the squamous epithelium of the esophagus.
TS25: esophagus	Present	Regionally restricted	6C	Moderate expression in the smooth muscle of the esophagus.

Structure	Level	Pattern	Image	Note
TS25: stomach	Present	Regionally restricted	6D	Expression in the squamous epithelium of the forestomach.

Structure	Level	Pattern	Image	Note
TS20: liver	Present	Regionally restricted		Expression in megakaryocytes.

Structure	Level	Pattern	Image	Note
TS21: liver	Absent			No expression in hepatocytes.

Structure	Level	Pattern	Image	Note
TS25: bronchiole	Present	Regionally restricted	8D	Low levels of expression in the epithelium of bronchioles. Higher levels of expression in the subepithelium at the base of epithelial cells.

Structure	Level	Pattern	Image	Note
TS22: lens fiber	Strong

Structure	Level	Pattern	Image	Note
TS25: lens fiber	Present		9A
TS25: lens epithelium	Absent		9A

Structure	Level	Pattern	Image	Note
TS25: lens equatorial epithelium	Absent		9B	No expression at the border between the lens fibers and lens epithelium.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Strong		11A	Intense expression in the epithelial layer of the skin over the entire body.
TS22: dermis	Absent		11A

Structure	Level	Pattern	Image	Note
TS25: epidermis	Present		11B
TS25: dermis	Absent		11B

Structure	Level	Pattern	Image	Note
TS25: vibrissa	Present	Regionally restricted	11D	Expression in whisker follicles.

Structure	Level	Pattern	Image	Note
TS25: vibrissa follicle	Present		11E