Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:31592
Mullen RJ, et al., NeuN, a neuronal specific nuclear protein in vertebrates. Development. 1992 Sep;116(1):201-11
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:3836190
|
Gene symbol:
|
Rbfox3
|
Gene name:
|
RNA binding protein, fox-1 homolog (C. elegans) 3
|
|
Results
|
Specimen
1A:
postnatal adult
(more )
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: brain |
Present |
Regionally restricted |
1A
|
Expression in nuclei of most neuronal cell types in all regions of the brain, including cerebral cortex, hippocampus, thalamus, caudate/putamen and cerebellum. Expression in cytoplasm usually markedly lower than in the nucleus.
|
Specimen
1B:
postnatal adult
(more )
Specimen
1B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Strong |
Regionally restricted |
1B
|
Higher magnification was shown in the inset. Intense expression in large pyramidal cells; less expression in the cytoplasm; no expression in the nucleolus.
|
TS28: neocortex layer |
Present |
Regionally restricted |
1B
|
Higher magnification was shown in the inset. Authors report expression in all cortical layers.
|
TS28: cerebral hemisphere white matter |
Absent |
|
1B
|
Higher magnification was shown in the inset.
|
TS28: corpus callosum |
Absent |
|
1B
|
Higher magnification was shown in the inset.
|
Specimen
1C:
postnatal adult
(more )
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2A,B:
postnatal adult
(more )
Note: Embedded in methacrylate.
Specimen
2A,B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embedded in methacrylate.
|
Specimen
cerebellum molecular layer - not shown:
postnatal adult
(more )
Specimen
cerebellum molecular layer - not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Specimen
heterozygous weaver mouse - not shown:
postnatal adult; Kcnj6wv/Kcnj6 +
(more )
Note: Known to have large numbers of ectopic granule cells.
Specimen
heterozygous weaver mouse - not shown:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Mutant Allele(s):
|
Kcnj6wv/Kcnj6+
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Note:
|
Known to have large numbers of ectopic granule cells.
|
Specimen
2C:
postnatal adult
(more )
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2D:
postnatal adult
(more )
Specimen
2D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3:
postnatal adult
(more )
Specimen
3:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
Present |
Regionally restricted |
3
|
Nuclear expression in large hippocampal neurons; no nuclear expression in astrocytes, or in glia within the neuronal layer.
|
Specimen
Pcd mouse - not shown:
postnatal adult; Agtpbp1pcd/ Agtpbp1pcd
(more )
Note: Known to have extensive proliferation of reactive glia in the molecular layer.
Specimen
Pcd mouse - not shown:
(close )
Genetic Background:
|
involves: C57BR/cdJ * CBA
|
Mutant Allele(s):
|
Agtpbp1pcd/Agtpbp1pcd
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Note:
|
Known to have extensive proliferation of reactive glia in the molecular layer.
|
Specimen
E9.5 - not shown:
embryonic day 9.5
(more )
Specimen
E9.5 - not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: nervous system |
Present |
|
|
Authors state that expression is confined to the nervous system.
|
Specimen
4A:
embryonic day 10.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
E10.5 spinal cord - not shown:
embryonic day 10.5
(more )
Specimen
E10.5 spinal cord - not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Regionally restricted |
|
Authors state that the more cranial regions of spinal cord have greater expression in ventral than in dorsal regions.
|
Specimen
4B,C:
embryonic day 12.5
(more )
Specimen
4B,C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
4C
,
4B
|
Greater expression in ventral than in dorsal regions; large numbers of expressing cells in ventral horns; expression in dorsal horns.
|
TS20: spinal cord floor plate |
Absent |
|
4C
,
4B
|
No expression in ventricular zone, roof plate, floor plate or marginal zone.
|
TS20: spinal cord roof plate |
Absent |
|
4C
,
4B
|
No expression in ventricular zone, roof plate, floor plate or marginal zone.
|
TS20: spinal cord ventricular layer |
Absent |
|
4C
,
4B
|
No expression in ventricular zone, roof plate, floor plate or marginal zone.
|
TS20: dorsal root ganglion |
Absent |
|
4B
,
4C
|
|
Specimen
4D:
embryonic day 17.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: telencephalon |
Present |
|
4D
|
Expression in cells throughout the cerebral hemispheres.
|
TS25: cortical subplate |
Strong |
Regionally restricted |
4D
|
In the neocortex, most intense expression in cells appearing as a single lamina whose position suggests these may be subplate (sublayer VIb) neurons.
|
TS25: cerebral cortex marginal zone |
Present |
Regionally restricted |
4D
|
Some expression in marginal layer which may be Cajal-Retzius cells.
|
TS25: piriform cortex |
Strong |
|
4D
|
Authors report strong expression across all layers.
|
Specimen
4E:
postnatal day 3
(more )
Specimen
4E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cerebellum granule cell layer |
Present |
Regionally restricted |
4E
|
Expression in cells along the inner margin of the external granular layer (post-mitotic cells before they migrate to the mature granular layer) as wells as cells as they migrate.
|
Specimen
adult - not shown:
postnatal adult
(more )
Specimen
adult - not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Paraffin
|
|