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Gene Expression Data
Assay Details
Assay
Reference: J:31592 Mullen RJ, et al., NeuN, a neuronal specific nuclear protein in vertebrates. Development. 1992 Sep;116(1):201-11
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3836190
Gene symbol: Rbfox3
Gene name: RNA binding protein, fox-1 homolog (C. elegans) 3
Antibody: Anti-NeuN (clone A60; MAB377)
Detection system: horseradish peroxidase
Results
Specimen 1A: postnatal adult (more )
Structure Level Pattern Image Note
TS28: brain Present Regionally restricted 1A Expression in nuclei of most neuronal cell types in all regions of the brain, including cerebral cortex, hippocampus, thalamus, caudate/putamen and cerebellum. Expression in cytoplasm usually markedly lower than in the nucleus.

Specimen 1B: postnatal adult (more )
Structure Level Pattern Image Note
TS28: cerebral cortex Strong Regionally restricted 1B Higher magnification was shown in the inset. Intense expression in large pyramidal cells; less expression in the cytoplasm; no expression in the nucleolus.
TS28: neocortex layer Present Regionally restricted 1B Higher magnification was shown in the inset. Authors report expression in all cortical layers.
TS28: cerebral hemisphere white matter Absent 1B Higher magnification was shown in the inset.
TS28: corpus callosum Absent 1B Higher magnification was shown in the inset.

Specimen 1C: postnatal adult (more )
Structure Level Pattern Image Note
TS28: spinal cord dorsal horn Present 1C
TS28: spinal cord ventral horn Present 1C
TS28: spinal cord white matter Absent 1C

Specimen 2A,B: postnatal adult (more )
Note: Embedded in methacrylate.
Structure Level Pattern Image Note
TS28: cerebellum granule cell layer Strong Regionally restricted 2A , 2B Intense nuclear expression in granule cells.
TS28: cerebellar cortex Strong Regionally restricted 2B , 2A Authors report strong expression in Golgi type II neurons and cells in the deep nuclei.
TS28: deep cerebellar nucleus Strong Regionally restricted 2B , 2A Authors report strong expression in Golgi type II neurons and cells in the deep nuclei.
TS28: cerebellum Purkinje cell layer Absent 2B , 2A No expression in Purkinje cells or in putative Bergmann glia.
TS28: cerebellum white matter Absent 2B , 2A No expression in Purkinje cells or in putative Bergmann glia.

Specimen cerebellum molecular layer - not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: cerebellum molecular layer Present Single cells

Specimen heterozygous weaver mouse - not shown: postnatal adult; Kcnj6wv/Kcnj6+ (more )
Note: Known to have large numbers of ectopic granule cells.
Structure Level Pattern Image Note
TS28: cerebellum molecular layer Present Authors report large numbers of immunoreactive cells in the molecular layer.

Specimen 2C: postnatal adult (more )
Structure Level Pattern Image Note
TS28: main olfactory bulb granule cell layer Strong Regionally restricted 2C Intense nuclear expression in granule cells.
TS28: main olfactory bulb mitral cell layer Absent 2C No expression in mitral cells.

Specimen 2D: postnatal adult (more )
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Strong 2D Intense expression of gangion cells.
TS28: retina inner nuclear layer Present Regionally restricted 2D Expression in some cells along the inner margin of the inner nuclear layer; no expression in most cells in the inner nuclear layer.
TS28: photoreceptor layer Absent 2D No expression in photoreceptor cells in the outer nuclear layer.
TS28: retina outer nuclear layer Absent 2D No expression in photoreceptor cells in the outer nuclear layer.

Specimen 3: postnatal adult (more )
Structure Level Pattern Image Note
TS28: hippocampus Present Regionally restricted 3 Nuclear expression in large hippocampal neurons; no nuclear expression in astrocytes, or in glia within the neuronal layer.

Specimen Pcd mouse - not shown: postnatal adult; Agtpbp1pcd/Agtpbp1pcd (more )
Note: Known to have extensive proliferation of reactive glia in the molecular layer.
Structure Level Pattern Image Note
TS28: cerebellum molecular layer Absent Authors report no immunoreactivity in reactive glia.

Specimen E9.5 - not shown: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: nervous system Present Authors state that expression is confined to the nervous system.

Specimen 4A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 4A Expression in cells at the lateral margins of the basal plate in areas with post-mitotic cells.
TS17: midbrain floor plate Absent 4A No expression in the ventricular zone and floor plate.
TS17: midbrain ventricular layer Absent 4A No expression in the ventricular zone and floor plate.

Specimen E10.5 spinal cord - not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted Authors state that the more cranial regions of spinal cord have greater expression in ventral than in dorsal regions.

Specimen 4B,C: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 4C , 4B Greater expression in ventral than in dorsal regions; large numbers of expressing cells in ventral horns; expression in dorsal horns.
TS20: spinal cord floor plate Absent 4C , 4B No expression in ventricular zone, roof plate, floor plate or marginal zone.
TS20: spinal cord roof plate Absent 4C , 4B No expression in ventricular zone, roof plate, floor plate or marginal zone.
TS20: spinal cord ventricular layer Absent 4C , 4B No expression in ventricular zone, roof plate, floor plate or marginal zone.
TS20: dorsal root ganglion Absent 4B , 4C

Specimen 4D: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: telencephalon Present 4D Expression in cells throughout the cerebral hemispheres.
TS25: cortical subplate Strong Regionally restricted 4D In the neocortex, most intense expression in cells appearing as a single lamina whose position suggests these may be subplate (sublayer VIb) neurons.
TS25: cerebral cortex marginal zone Present Regionally restricted 4D Some expression in marginal layer which may be Cajal-Retzius cells.
TS25: piriform cortex Strong 4D Authors report strong expression across all layers.

Specimen 4E: postnatal day 3 (more )
Structure Level Pattern Image Note
TS27: cerebellum granule cell layer Present Regionally restricted 4E Expression in cells along the inner margin of the external granular layer (post-mitotic cells before they migrate to the mature granular layer) as wells as cells as they migrate.

Specimen adult - not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: cerebellum granule cell layer Present Regionally restricted Expression in granular layer is more cytoplasmic than nuclear.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory