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Gene Expression Data
Assay Details
Assay
Reference: J:90391 Yu K, et al., Conditional inactivation of FGF receptor 2 reveals an essential role for FGF signaling in the regulation of osteoblast function and bone growth. Development. 2003 Jul;130(13):3063-74
Assay type: Recombinase reporter
MGI Accession ID: MGI:3835393
Gene symbol: Twist2
Gene name: twist basic helix-loop-helix transcription factor 2
Reporter: lacZ
Direct Detection
Results
Specimen not shown: embryonic day 9.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1(cre)Dor/Twist2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: surface ectoderm Present Staining is observed at surface of embryo.
Specimen Fig. 1D: embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1(cre)Dor/Twist2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: branchial arch Present 1D
TS19: somite Present 1D
Specimen Fig. 1E: embryonic day 15.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. This is a sagittal section through the embryo.
Structure Level Pattern Image Note
TS23: embryo Present Regionally restricted 1E Staining is not observed in ectodermal tissues.
Specimen Fig. 1F: embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. This is a paraffin section of a limb bud.
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present 1F Mesenchymal condensations in the forelimb bud exhibit staining.
TS19: forelimb bud ectoderm Absent 1F Ectodermal epithelium of the forelimb bud does not stain.
Specimen Fig. 1G: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1(cre)Dor/Twist2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: femur Present 1G Perichondrium and periosteum are uniformly stained in the femur, but there are some unlabeled chondrocytes apparent.
Specimen Fig. 1I: postnatal adult; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1(cre)Dor/Twist2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: tibia Present Regionally restricted 1I Staining is observed in the growth plate of the tibia.
Specimen Fig. 1J: postnatal adult; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1(cre)Dor/Twist2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: tibia Present Regionally restricted 1J Staining is observed in the cortical bone.
TS28: bone Present Regionally restricted 1J Staining is observed in the perichondrium, periosteum and endosteum.
TS28: cartilage Present Regionally restricted 1J Staining is observed in chondrocytes in growth plate cartilage. Occasionally a clone of chondrocytes that fails to stain for B-gal activity is observed.
Specimen Fig. 1L: postnatal adult; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Twist2tm1(cre)Dor/Twist2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: bone Present 1L Staining is observed in osteoblasts lining the bone trabeculae. There is no staining in the osteoclasts.
TS28: tibia Present Uniform 1L Perichondrium and periosteum are uniformly stained.
TS28: bone marrow Absent 1L
TS28: hindlimb musculature Absent 1L
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory