Gene Expression Data
Assay Details
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Assay
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Reference:
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J:143598
Jaks V, et al., Lgr5 marks cycling, yet long-lived, hair follicle stem cells. Nat Genet. 2008 Nov;40(11):1291-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3832657
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Gene symbol:
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Mki67
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Gene name:
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antigen identified by monoclonal antibody Ki 67
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Antibody:
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anti-Ki67 Antigen
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to I125
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Assay notes:
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Specimens were fixed in 2% paraformaldehyde and 0.2% glutaraldehyde.
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Results
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Specimen
2a:
(close  )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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postnatal week 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Telogen phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
2a
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Expression in the upper portion of the hair follicle isthmus. No expression in the bulge area.
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Specimen
2b:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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postnatal week 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Pre-anagen phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
2b
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Some expression in the secondary germ area.
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Specimen
2c:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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postnatal week 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Early anagen phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
2c
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Expression in the secondary germ area.
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Specimen
2d:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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postnatal week 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Anagen phase.
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Specimen
2f:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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postnatal week 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Anagen phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
2f
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Expression concentrated in the bulb, both in the outer cell layer and in the matrix.
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Specimen
2g:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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postnatal week 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Anagen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
2g
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Expression concentrated in the bulb, both in the outer cell layer and in the matrix.
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Specimen
2h:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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postnatal week 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Late anagen phase.
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Specimen
2i:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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postnatal week 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Late anagen phase.
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Analysis Tools
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