Probe:	pGEMte-mPcdh1-ISH
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS16: blood vessel	Present		2A	Shown is the mandibular arch.
TS16: common atrial chamber endocardial lining	Present		2A	Expression is in the endothelial lining of the heart.

Structure	Level	Pattern	Image	Note
TS23: blood vessel	Present		2C	Shown is a toe with expression in small blood vessels.

Structure	Level	Pattern	Image	Note
TS23: blood vessel	Present		2D	Expression is in the blood vessels of the compact (outer) part of the ventricular myocardium, but the endothelial lining of the trabeculated (inner) part does not.
TS23: heart ventricle	Present		2D	Expression is in the blood vessels of the compact (outer) part of the ventricular myocardium, but the endothelial lining of the trabeculated (inner) part does not.

Structure	Level	Pattern	Image	Note
TS28: blood vessel	Weak		2E

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong		2G	Expression is in the endothelial lining of the sinusoids.

Structure	Level	Pattern	Image	Note
TS16: extraembryonic component	Present	Regionally restricted	2H	Expression is in some trophoblast cells within the spongiotrophoblast and labyrinthine layer of the chorioallantoic placenta.
TS28: placenta	Present	Regionally restricted	2H	Expression is in some trophoblast cells within the spongiotrophoblast and labyrinthine layer of the chorioallantoic placenta.

Structure	Level	Pattern	Image	Note
TS20: yolk sac	Strong	Regionally restricted	2I	Expression is in the epithelium.
TS20: extraembryonic component	Weak	Regionally restricted	2I	Weak to moderate expression is in the Reichert's membrane.

Structure	Level	Pattern	Image	Note
TS21: extraembryonic component	Present	Regionally restricted	2J	Expression is in glycogen cells within the spongiotrophoblast layer and the labyrinth is negative.
TS28: placenta	Present	Regionally restricted	2J	Expression is in glycogen cells within the spongiotrophoblast layer and the labyrinth is negative.

Structure	Level	Pattern	Image	Note
TS23: blood vessel	Present		2K	Expression is in the developing blood vessels of the renal cortex.
TS23: renal cortex	Present		2K	Expression is in the developing blood vessels of the renal cortex.

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Strong		2M
TS28: renal cortex	Moderate	Regionally restricted	2M	Expression is in epithelial components.
TS28: renal medulla	Moderate	Regionally restricted	2M	Moderate expression in the tubular system, absent in blood vessels.

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Present		2N
TS28: renal proximal tubule	Present		2N
TS28: renal distal tubule	Absent		2N
TS28: renal medulla	Absent		2N

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Present		2O
TS28: renal proximal tubule	Present		2O

Structure	Level	Pattern	Image	Note
TS21: midgut	Present	Regionally restricted	2Q	Expression is in the epithelial lining of the midgut intestinal mucosa and by scattered cells in the submucosa.

Structure	Level	Pattern	Image	Note
TS23: midgut	Present	Regionally restricted	2R	Expression is in the epithelial lining of the midgut intestinal mucosa and by scattered cells in the submucosa.

Structure	Level	Pattern	Image	Note
TS23: esophagus	Present	Regionally restricted	2S	Expression is at the transition between esophagus and stomach.
TS23: stomach	Present	Regionally restricted	2S	Expression is at the transition between esophagus and stomach.
TS23: esophagus epithelium	Present		2S
TS23: stomach epithelium	Absent		2S

Structure	Level	Pattern	Image	Note
TS23: midgut	Present	Regionally restricted	2T	Expression is in the epithelial lining of the midgut intestinal mucosa and by scattered cells in the submucosa.

Structure	Level	Pattern	Image	Note
TS28: small intestine crypt of lieberkuhn	Strong		2V
TS28: intestinal villus	Present		2V

Structure	Level	Pattern	Image	Note
TS23: lung	Present	Regionally restricted	2W	Expression is in the parenchyma, and is absent in the bronchi.
TS23: liver	Present		2W

Structure	Level	Pattern	Image	Note
TS25: heart left ventricle	Present	Regionally restricted		Expression is in small blood vessels.
TS25: heart right ventricle	Present	Regionally restricted		Expression is in small blood vessels.
TS25: endocardial tissue	Absent			Expression is absent in the endothelial lining of the heart.
TS28: placenta	Weak
TS25: peripheral nervous system	Present			Expression is in most neurons in the spinal ganglia, cranial sensory ganglia, and visceral ganglia form the early stages of embryogenesis (E10-E12) to postnatal stages.
TS25: glomerular tuft	Strong
TS25: esophagus epithelium	Present
TS25: stomach epithelium	Absent
TS25: blood vessel	Strong	Regionally restricted		Expression is in thymic blood vessels.
TS25: thymus	Strong	Regionally restricted		Expression is in thymic blood vessels.

Structure	Level	Pattern	Image	Note
TS16: yolk sac	Present
TS16: floor plate	Present
TS16: peripheral nervous system	Present	Regionally restricted		Expression is in most neurons in the spinal ganglia, cranial sensory ganglia, and visceral ganglia form the early stages of embryogenesis (E10-E12) to postnatal stages.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present	Regionally restricted		Expression is in some cellular aggregates and epithelial structures are negative.
TS21: medulla oblongata alar plate marginal layer	Present
TS21: medulla oblongata basal plate marginal layer	Present
TS21: peripheral nervous system	Present	Regionally restricted		Expression is in most neurons in the spinal ganglia, cranial sensory ganglia, and visceral ganglia form the early stages of embryogenesis (E10-E12) to postnatal stages.

Structure	Level	Pattern	Image	Note
TS28: urothelium of bladder	Absent

Structure	Level	Pattern	Image	Note
TS28: lung	Present	Regionally restricted	3A	Expression is in the parenchyma, and is absent in the bronchi.

Structure	Level	Pattern	Image	Note
TS28: liver parenchyma	Present		3B

Structure	Level	Pattern	Image	Note
TS25: submandibular gland epithelium	Present	Ubiquitous	3C
TS25: blood vessel	Present	Regionally restricted	3C	Ubiquitous expression in the blood vessels of the submandibular gland.

Structure	Level	Pattern	Image	Note
TS28: endometrium	Present		3F

Structure	Level	Pattern	Image	Note
TS28: epithelium of vagina	Present		3G

Structure	Level	Pattern	Image	Note
TS28: myometrium	Present		3H	Expression is in the tubular glands.
TS28: endometrium	Absent		3H
TS28: ovary	Absent		3H

Structure	Level	Pattern	Image	Note
TS28: prostate epithelium	Present		3I

Structure	Level	Pattern	Image	Note
TS28: epithelium of seminal vesicle	Present		3J

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		3K

Structure	Level	Pattern	Image	Note
TS28: male reproductive system	Absent

Structure	Level	Pattern	Image	Note
TS23: alar columns	Strong	Regionally restricted	3L	Expression is in the superficial layers of the dorsal column.
TS23: spinal ganglion	Present		3L
TS23: sympathetic ganglion	Present		3L

Structure	Level	Pattern	Image	Note
TS23: basal columns	Present	Regionally restricted	3L inset	Expression is present at some spinal cord levels.

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Present	Regionally restricted		Expression is strong in the superficial layers of the dorsal column and is present in the motor column at some spinal cord levels.
TS28: vomeronasal organ	Present

Structure	Level	Pattern	Image	Note
TS21: blood vessel	Present	Regionally restricted	3M	Expression is in vessels in and around the brain.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong		3N
TS28: indusium griseum	Strong		3N
TS28: amygdala	Present		3N
TS28: cortical layer V	Present		3N
TS28: dorsal lateral geniculate nucleus	Present		3N
TS28: substantia nigra	Present		3N
TS28: ventral lateral geniculate nucleus	Present		3N
TS28: cortical layer I	Weak		3N
TS28: cortical layer II	Weak		3N
TS28: cortical layer III	Weak		3N
TS28: cortical layer IV	Weak		3N
TS28: cortical layer VI	Weak		3N
TS28: dentate gyrus	Weak		3N

Structure	Level	Pattern	Image	Note
TS23: retina	Present	Regionally restricted		Expression is in the prospective ganglion cell layer and inner nuclear layer.
TS23: vomeronasal organ	Present

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		3O
TS28: retina inner nuclear layer	Present		3O

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Present		3P
TS28: main olfactory bulb glomerular layer	Present		3P
TS28: main olfactory bulb granule cell layer	Present		3P

Structure	Level	Pattern	Image	Note
TS26: vomeronasal organ	Present		3Q

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Present		3R
TS28: organ of Corti	Present	Regionally restricted	3R	Expression is in superficial cells.

Structure	Level	Pattern	Image	Note
TS26: hair follicle	Present		3S	Expression is in all layers of the hair bulb and is weak in the dermal papilla. It is strong in the inner rooth sheath and absent in the outer root sheath.

Structure	Level	Pattern	Image	Note
TS28: hair bulb	Present		3T	Expression is in all layers of the hair bulb and is weak in the dermal papilla.
TS28: hair inner root sheath	Strong		3T
TS28: hair outer root sheath	Absent		3T

Structure	Level	Pattern	Image	Note
TS23: thymus primordium	Present	Regionally restricted	3U	Expression is in the endothelial lining of the sinuses.

Structure	Level	Pattern	Image	Note
TS28: blood vessel	Weak	Regionally restricted		Expression is in thymic blood vessels.
TS28: thymus	Weak	Regionally restricted		Expression is in thymic blood vessels.

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Present		3W
TS26: retina nuclear layer	Present	Regionally restricted	3W	Expression is in the ganglion cell layer.
TS26: nasal meatus	Present	Regionally restricted	3W	Expression is in the glandular epithelium of serous glands in the lateral wall of the middle meatus.

Structure	Level	Pattern	Image	Note
TS26: nasal meatus	Present	Regionally restricted	3X	Expression is in the glandular epithelium of serous glands in the lateral wall of the middle meatus.

Structure	Level	Pattern	Image	Note
TS26: nasal cavity epithelium	Strong	Regionally restricted	3Y	Expression in the most superficial layer is absent whereas the deeper layer shows a very strong signal.