Probe:	Dlx5 probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS14: head mesenchyme	Present	Regionally restricted	3a	Expression in facial mesenchyme.
TS14: 1st branchial arch mandibular component mesenchyme	Strong		3a
TS14: 1st branchial arch maxillary component mesenchyme	Strong		3a
TS14: 2nd branchial arch mesenchyme	Weak		3a	Slightly weaker expression in hyoid arch.
TS14: branchial arch mesenchyme	Weak	Regionally restricted	3a	Weak expression in third and fourth branchial arches.
TS14: otic placode	Present		3a	Authors report expression in the otic vesicle.
TS14: embryo ectoderm	Present	Regionally restricted	3a	Expression in frontonasal ectoderm around olfactory placodes.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	3c	Expression in developing forebrain in primordia of the ganglionic eminence and ventral diencephalic regions.
TS17: telencephalon	Present	Regionally restricted	3c	Expression in developing forebrain in primordia of the ganglionic eminence and ventral diencephalic regions.
TS17: optic recess	Absent		3c	No expression in region of preoptic recess.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	4a	Domain extends anteriorly away from zona limitans intrathalamica, separating ventral from dorsal thalamus, to postoptic area; includes posterior entopeduncular area, hypothalamic cells cord, and primordia of suprachiasmatic area.
TS20: telencephalon	Present	Regionally restricted	4a	Domain extends away from preoptic area along floor of cerebral vesicle, under primordia of medial and lateral ganglionic eminence, and ends at boundary between ganglionic eminence and telencephalic cortical regions.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	4b	Gap of expression in region of optic stalk between preoptic and suprachiasmatic areas which gives rise to the eminentia thalami and several hypothalamic primordia.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4e	Expression abruptly stopped at boundary between ganglionic eminence and cortical regions of dorsal telencephalon.
TS20: cranium	Present		4e	Expression in developing skull bones.

Structure	Level	Pattern	Image	Note
TS20: skeleton	Present		4f	Expression in practically every developing skeletal element, starting from first cartilage condensation.
TS20: axial skeleton	Present	Regionally restricted	4f	Expression in perichondrial areas of developing vertebrae, ribs, long and short bones of trunk and limbs, and craniofacial cartilagenous and skeletal structures.
TS20: cranium	Present	Regionally restricted	4f	Expression in perichondrial areas of developing vertebrae, ribs, long and short bones of trunk and limbs, and craniofacial cartilagenous and skeletal structures.
TS20: limb	Present	Regionally restricted	4f	Expression in perichondrial areas of developing vertebrae, ribs, long and short bones of trunk and limbs, and craniofacial cartilagenous and skeletal structures.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	4j	In ventral brain regions, expression in ventral thalamus and basal regions; slightly higher in a subventricular stripe of cells.
TS20: thalamus	Present	Regionally restricted	4j	Sharp dorsal boundary at level of boundary between dorsal and ventral thalamus-the zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present		5a

Structure	Level	Pattern	Image	Note
TS23: brain	Present		5b	Expression in both brain and skeleton essentially unaltered from previous stages.
TS23: skeleton	Present		5b	Expression in both brain and skeleton essentially unaltered from previous stages.

Structure	Level	Pattern	Image	Note
TS25: embryo	Weak		5c	Authors state expression slowly decreased.

Structure	Level	Pattern	Image	Note
TS25: tooth	Present		5d