Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:139954
Simmons DG, et al., Spatial and temporal expression of the 23 murine Prolactin/Placental Lactogen-related genes is not associated with their position in the locus. BMC Genomics. 2008;9:352
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3820700
|
|
Gene symbol:
|
Prl4a1
|
|
Gene name:
|
prolactin family 4, subfamily a, member 1
|
|
Probe:
|
Prl4a1 cDNA1
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
There is a slight strain difference in expression as more CTGCs (canal trophoblast giant cell) appear to be expressing Prl4a1 in C57BL/6 and 129svJ backgrounds than in the CD-1 background.
|
|
|
Results
|
Specimen
3 Prl4a1:
embryonic day 8.5
(more  )
Specimen
3 Prl4a1:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
Prl4a1 E8.5:
embryonic day 8.5
(more )
Specimen
Prl4a1 E8.5:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
Prl4a1 E14.5:
embryonic day 14.5
(more )
Specimen
Prl4a1 E14.5:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: extraembryonic component |
Present |
Regionally restricted |
Additional File Prl4a1_A E14.5
|
Expression is in spongiotrophoblasts and in glycogen trophoblast cells and is absent in the chorion or in syncytiotrophoblast of the labyrinth layer. Expression in and around the spiral arteries remains is high.
|
| TS28: placenta |
Present |
Regionally restricted |
Additional File Prl4a1_A E14.5
|
Expression is in spongiotrophoblasts and in glycogen trophoblast cells and is absent in the chorion or in syncytiotrophoblast of the labyrinth layer. Expression in and around the spiral arteries remains is high.
|
Specimen
Not Shown C57BL/6:
embryonic day 14.5
(more )
Specimen
Not Shown C57BL/6:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: extraembryonic component |
Present |
Regionally restricted |
|
More CTGCs (canal trophoblast giant cell) appear to be expressing Prl4a1 in C57BL/6 and 129svJ backgrounds than in the CD-1 background.
|
| TS28: placenta |
Present |
Regionally restricted |
|
More CTGCs (canal trophoblast giant cell) appear to be expressing Prl4a1 in C57BL/6 and 129svJ backgrounds than in the CD-1 background.
|
Specimen
Not Shown 129/SvJ:
embryonic day 14.5
(more )
Specimen
Not Shown 129/SvJ:
(close )
|
Genetic Background:
|
129X1/SvJ
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: extraembryonic component |
Present |
Regionally restricted |
|
More CTGCs (canal trophoblast giant cell) appear to be expressing Prl4a1 in C57BL/6 and 129svJ backgrounds than in the CD-1 background.
|
| TS28: placenta |
Present |
Regionally restricted |
|
More CTGCs (canal trophoblast giant cell) appear to be expressing Prl4a1 in C57BL/6 and 129svJ backgrounds than in the CD-1 background.
|
|
Analysis Tools