Antibody:

anti-Twist

Structure	Level	Pattern	Image	Note
TS21: palatal shelf epithelium	Present	Single cells	1B , 1A	Expression was present only in a few cells.
TS21: palatal shelf mesenchyme	Present		1A , 1B

Structure	Level	Pattern	Image	Note
TS22: palatal shelf	Present	Non-Uniform	1C , 1D	There is increased staining at the tip of the palatal shelves, where the midline epithelial seam will form.

Structure	Level	Pattern	Image	Note
TS23: primary palate epithelium	Present	Non-Uniform	1E , 1F	Expression is downregulated from what was seen earlier but scattered islands of expressing cells are still seen, including in the epithelial triangle.
TS23: primary palate mesenchyme	Present		1F , 1E

Structure	Level	Pattern	Image	Note
TS23: nasal cavity epithelium	Present
TS23: oral epithelium	Present

Structure	Level	Pattern	Image	Note
TS24: secondary palate	Present	Single cells	1H , 1G	Expression is present in a few cells in the mesenchyme.