Gene Expression Data
Assay Details
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Assay
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Reference:
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J:29276
Acampora D, et al., Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation. Development. 1995 Oct;121(10):3279-90
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3810316
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Reporter:
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lacZ
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Probe:
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lacZ probe2
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Probe preparation:
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labelled with S35
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Visualized with:
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Autoradiography
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Results
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Specimen
2c:
embryonic day 5.75; Otx2tm1Pas/Otx2 +
(more  )
Specimen
2c:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2+
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Age:
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embryonic day 5.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2h:
embryonic day 6.5; Otx2tm1Pas/Otx2 +
(more )
Specimen
2h:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2+
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo ectoderm |
Present |
Regionally restricted |
2h
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Expression is in the external layer with a progressive anteriorization.
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Specimen
2m:
embryonic day 7.75; Otx2tm1Pas/Otx2 +
(more )
Note: Head-fold stage.
Specimen
2m:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2+
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Head-fold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo ectoderm |
Present |
Regionally restricted |
2m
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Expression is in the anterior part of all three germ layers.
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| TS11: embryo endoderm |
Present |
Regionally restricted |
2m
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Expression is in the anterior part of all three germ layers.
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| TS11: embryo mesoderm |
Present |
Regionally restricted |
2m
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Expression is in the anterior part of all three germ layers.
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Specimen
5e:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2tm1Pas
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo endoderm |
Present |
Regionally restricted |
5e
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Expression is in the external layer containing endomesoderm cells in the distal third to half.
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| TS9: embryo mesoderm |
Present |
Regionally restricted |
5e
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Expression is in the external layer containing endomesoderm cells in the distal third to half.
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| TS9: embryo ectoderm |
Weak |
Regionally restricted |
5e
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Expression is in the distal third to half.
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Specimen
6d:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2tm1Pas
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools
