Gene Expression Data
Assay Details
Assay
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Reference:
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J:62506
Hemberger M, et al., cDNA subtraction cloning reveals novel genes whose temporal and spatial expression indicates association with trophoblast invasion. Dev Biol. 2000 Jun 1;222(1):158-69
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3807475
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Gene symbol:
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Cts7
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Gene name:
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cathepsin 7
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Probe:
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Epcs24
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Probe preparation:
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labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Fixative: Serra's fixative.
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Results
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Specimen
5A:
embryonic day 7.5
(more )
Specimen
5A:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 7.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS11: primary trophoblast giant cell |
Strong |
Regionally restricted |
5A
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Expression was abundant in a subset of trophoblast giant cells in the parietal yolk sac and ectoplacental cone.
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Specimen
5B:
embryonic day 7.5
(more )
Specimen
5B:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS11: primary trophoblast giant cell |
Strong |
Regionally restricted |
5B
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Expression was abundant in a subset of trophoblast giant cells in the parietal yolk sac and ectoplacental cone.
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Specimen
5D & E:
embryonic day 15.5
(more )
Note: Fig. 5D is the bright-field image and 5E is the dark-field image.
Specimen
5D & E:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Fig. 5D is the bright-field image and 5E is the dark-field image.
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Structure |
Level |
Pattern |
Image |
Note |
TS28: placenta |
Present |
Regionally restricted |
5D/E
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Expression was detected in trophoblast cells within the labyrinthine region. Signal was absent from spongiotrophoblast and trophoblast giant cells.
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TS28: decidua |
Absent |
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5D/E
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