Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS28: impulse conducting system	Present	Regionally restricted	1E	Intense signals were detected in the cardiac conduction system from the atrioventricular node to the bundle branches.
TS28: cardiac muscle tissue	Present	Regionally restricted	1E	Expression was detected occasionally at the working cardiac muscle cells.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Widespread	3B
TS13: neural tube	Present		3B

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Present		3D
TS15: branchial arch	Present		3D
TS15: future forebrain	Present		3D
TS15: limb	Present		3D
TS15: neural tube	Present		3D
TS15: outflow tract	Present		3D
TS15: trunk somite	Present		3D

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		3E	Expression pattern was similar to the heterozygous embryo.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		3F

Structure	Level	Pattern	Image	Note
TS13: cardiac muscle tissue	Weak	Regionally restricted	4A	Expression was detected in cardiac myocytes.
TS13: heart	Weak	Regionally restricted	4A	Expression was detected in cardiac endothelial cells and myocytes.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		4B
TS15: trunk somite	Present		4B
TS15: atrioventricular canal	Present	Regionally restricted	4B	Expression was detected in the endothelium lining the atrioventricular canal and the outflow tract.
TS15: outflow tract	Present	Regionally restricted	4B	Expression was detected in the endothelium lining the atrioventricular canal and the outflow tract.

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Present	Regionally restricted	4C	Expression was detected in the endothelium lining the atrioventricular canal and the outflow tract.
TS15: outflow tract	Present	Regionally restricted	4C	Expression was detected in the endothelium lining the atrioventricular canal and the outflow tract.

Structure	Level	Pattern	Image	Note
TS15: endocardial cushion	Present		4D

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Present	Regionally restricted	4E	Expression was detected in the endothelium lining the atrioventricular canal and the outflow tract.
TS15: outflow tract	Present	Regionally restricted	4E	Expression was detected in the endothelium lining the atrioventricular canal and the outflow tract.

Structure	Level	Pattern	Image	Note
TS15: endocardial cushion	Present		4F

Structure	Level	Pattern	Image	Note
TS17: endocardial cushion	Trace		4G	Signal in the endocardial cushion and the endothelium were negative, or very weak, as there was a trace of blue located in the cytoplasm.
TS17: atrioventricular canal	Trace	Regionally restricted	4G	Signal in the endocardial cushion and the endothelium were negative, or very weak, as there was a trace of blue located in the cytoplasm.
TS17: outflow tract	Trace	Regionally restricted	4G	Signal in the endocardial cushion and the endothelium were negative, or very weak, as there was a trace of blue located in the cytoplasm.

Structure	Level	Pattern	Image	Note
TS19: endocardial cushion	Trace		4H	Signal in the endocardial cushion and the endothelium were negative, or very weak, as there was a trace of blue located in the cytoplasm.
TS19: atrioventricular canal	Trace	Regionally restricted	4H	Signal in the endocardial cushion and the endothelium were negative, or very weak, as there was a trace of blue located in the cytoplasm.
TS19: outflow tract	Trace	Regionally restricted	4H	Signal in the endocardial cushion and the endothelium were negative, or very weak, as there was a trace of blue located in the cytoplasm.