Gene Expression Data
Assay Details
Assay
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Reference:
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J:20965
Timmons PM, et al., Expression and function of Pax 1 during development of the pectoral girdle. Development. 1994 Oct;120(10):2773-85
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3797922
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Gene symbol:
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Col2a1
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Gene name:
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collagen, type II, alpha 1
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Results
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Specimen
1B:
embryonic day 10.5
(more )
Specimen
1B:
(close )
Genetic Background:
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CBA/Ca
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Age:
|
embryonic day 10.5
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Sex:
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Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk mesenchyme |
Present |
Regionally restricted |
1B
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Expression was detected in a large patch of chondrogenic mesenchyme corresponding to the early scapulo-humeral blastema.
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Specimen
2B/B':
embryonic day 11.5-12.5
(more )
Note: Stage 5 of forelimb development.
Specimen
2B/B':
(close )
Genetic Background:
|
CBA/Ca
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Age:
|
embryonic day 11.5-12.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
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Stage 5 of forelimb development.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: shoulder mesenchyme |
Present |
Regionally restricted |
2B/B'
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Expression was detected in the scapulo-humeral blastema.
|
Specimen
not shown:
embryonic day 11.5-12.5
(more )
Note: Stage 6 of forelimb development.
Specimen
not shown:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.5-12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Stage 6 of forelimb development.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: shoulder mesenchyme |
Weak |
Regionally restricted |
|
Expression was weak in the condensing mesenchyme of the developing acromion of the spina scapula.
|
Specimen
2C:
embryonic day 11.5-12.5
(more )
Note: Stage 7 of forelimb development.
Specimen
2C:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.5-12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Stage 7 of forelimb development.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: shoulder mesenchyme |
Present |
Regionally restricted |
2C
|
Expression was strong in the blastemal scapula blade and spina. Expression was weak in the condensing mesenchyme of the developing acromion.
|
Specimen
2E/E':
embryonic day 11.5-12.5
(more )
Note: Stage 7 of forelimb development.
Specimen
2E/E':
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.5-12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Stage 7 of forelimb development.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: shoulder mesenchyme |
Present |
Regionally restricted |
2E/E'
|
Expression was strong in the blastemal scapula blade and spina. Expression was weak in the condensing mesenchyme of the developing acromion.
|
Specimen
2G:
embryonic day 11.5-12.5
(more )
Note: Stage 9 of forelimb development.
Specimen
2G:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.5-12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Stage 9 of forelimb development.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: shoulder mesenchyme |
Strong |
Regionally restricted |
2G
|
Expression was strong in the precartilaginous proximal end of the acromion.
|
Specimen
2J:
embryonic day 11.5-12.5
(more )
Note: Stage 9 of forelimb development.
Specimen
2J:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.5-12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Stage 9 of forelimb development.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: shoulder mesenchyme |
Weak |
Regionally restricted |
2J
|
Expression was weak in the distal (ventral) extremity of the acromion, where the cells were still undergoing condensation.
|
Specimen
3B:
embryonic day 11.5
(more )
Note: Stage 6 of forelimb development.
Specimen
3B:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Stage 6 of forelimb development.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hip mesenchyme |
Weak |
Regionally restricted |
3B
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Expression could just be visualized in the early blastemas of the ischium, ileum and pubis in the pelvic region.
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