Probe:	Dusp9 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS14: otic pit	Present

Structure	Level	Pattern	Image	Note
TS14: otic pit	Present	Regionally restricted	1H	Expression is in the anterior domain.

Structure	Level	Pattern	Image	Note
TS14: otic pit	Present	Regionally restricted	1H1	Expression is in the anterior domain.

Structure	Level	Pattern	Image	Note
TS15: branchial arch mesenchyme	Strong		1H2

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	1I	Expression is in the anterior domain.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	1J	Expression is concentrated laterally, approximately midway between the dorsal and ventral otic poles.
TS15: branchial arch mesenchyme	Strong		1J

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	1J1	Expression is concentrated laterally, approximately midway between the dorsal and ventral otic poles.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	1K	Expression is in mesenchyme immediately adjacent to the dorsal half of the vesicle that is forming the vertical canal plate.
TS17: branchial arch mesenchyme	Strong	Regionally restricted	1K	The highest levels are concentrated in the most distal reaches of the arches.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	1K1	Expression is in mesenchyme immediately adjacent to the dorsal half of the vesicle that is forming the vertical canal plate.
TS17: 2nd branchial arch mesenchyme	Strong	Regionally restricted	1K1	The highest levels are concentrated in the most distal reaches of the arch.

Structure	Level	Pattern	Image	Note
TS16: otocyst	Absent			Expression in the otic vesicle was no longer detectable after the cup closed entirely.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct mesenchyme	Present	Regionally restricted	2H	Expression is in the otic mesenchyme adjacent to the developing sensory epithelium.
TS22: cochlear duct epithelium	Absent		2H

Structure	Level	Pattern	Image	Note
TS22: cochlear duct mesenchyme	Present	Regionally restricted	2H1	Expression is in the otic mesenchyme adjacent to the developing sensory epithelium.
TS22: cochlear duct epithelium	Absent		2H1

Structure	Level	Pattern	Image	Note
TS24: cochlear duct mesenchyme	Present	Regionally restricted	2I	Expression is in the otic mesenchyme adjacent to the developing sensory epithelium.
TS24: cochlear duct epithelium	Absent		2I

Structure	Level	Pattern	Image	Note
TS24: cochlear duct mesenchyme	Present	Regionally restricted	2I1	Expression is in the otic mesenchyme adjacent to the developing sensory epithelium.
TS24: cochlear duct epithelium	Absent		2I1

Structure	Level	Pattern	Image	Note
TS22: external auditory meatus	Present	Regionally restricted	2L	Expression is in dispersed cells in the ectodermal layer.
TS22: pinna surface epithelium	Present	Regionally restricted	2L	Expression is in dispersed cells in the ectodermal layer.