Probe:	Dusp6 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Present	Regionally restricted	1A	Expression is in presumptive neural crest migrating lateral to the developing otic vesicles.

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Present	Regionally restricted	1A1	Expression is in presumptive neural crest migrating lateral to the developing otic vesicles.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme derived from neural crest	Present		1B
TS17: 2nd branchial arch mesenchyme derived from neural crest	Present		1B

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme derived from neural crest	Present		1B1
TS17: 2nd branchial arch mesenchyme derived from neural crest	Present		1B1

Structure	Level	Pattern	Image	Note
TS18: 1st branchial arch maxillary component mesenchyme derived from neural crest	Present		1C
TS18: 1st branchial groove ectoderm	Present		1C
TS18: 2nd branchial arch mesenchyme derived from neural crest	Present		1C
TS18: migrating neural crest	Present	Regionally restricted	1C	Expression is in presumptive neural crest migrating lateral to the developing otic vesicles.
TS18: head mesenchyme	Present	Regionally restricted	1C	Expression is in the mesenchyme adjacent to the otic vesicle.
TS18: 1st branchial arch	Present	Regionally restricted	1C	Expression is in the mesenchyme immediately adjacent to the first branchial cleft.

Structure	Level	Pattern	Image	Note
TS18: 1st branchial arch mandibular component mesenchyme derived from neural crest	Present		1C1
TS18: 1st branchial arch maxillary component mesenchyme derived from neural crest	Present		1C1
TS18: migrating neural crest	Present	Regionally restricted	1C1	Expression is in presumptive neural crest migrating lateral to the developing otic vesicles.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present	Regionally restricted	1D	Expression is in the mesenchyme immediately adjacent to the first branchial cleft.
TS19: 1st branchial groove epithelium	Present		1D
TS19: head mesenchyme	Present	Regionally restricted	1D	Expression is in the mesenchyme adjacent to the otic vesicle.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present	Regionally restricted	1D1	Expression is in the mesenchyme immediately adjacent to the first branchial cleft.
TS19: 1st branchial groove epithelium	Present		1D1
TS19: head mesenchyme	Present	Regionally restricted	1D1	Expression is in the mesenchyme adjacent to the otic vesicle.
TS19: inner ear epithelium	Absent		1D1

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present		2A1
TS20: inner ear vestibular component	Present		2A1	Expression is in the mesenchyme immediately adjacent to the developing Reissner's membrane.

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Strong		2A2	Strong expression in the spiral ganglion and less in other restricted areas.

Structure	Level	Pattern	Image	Note
TS20: saccule	Present		2A3

Structure	Level	Pattern	Image	Note
TS20: semicircular canal	Present	Regionally restricted	2A4	Expression is in the cristae of each semicircular canal.
TS20: inner ear vestibular component	Present	Regionally restricted	2A4	Expression is in the mesenchyme immediately adjacent to the developing Reissner's membrane and near the semicircular canal ducts.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present	Regionally restricted	2B1
TS22: inner ear vestibular component	Present	Regionally restricted	2B1	Expression is in the mesenchyme immediately adjacent to the developing Reissner's membrane.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Strong	Regionally restricted	2B2	Strong expression in the spiral ganglion and less in other restricted areas.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present		2B3

Structure	Level	Pattern	Image	Note
TS22: semicircular canal	Present	Regionally restricted	2B4	Expression is in the cristae of the semicircular canals.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct epithelium	Present	Regionally restricted	2C1

Structure	Level	Pattern	Image	Note
TS24: cochlear duct epithelium	Strong	Regionally restricted	2C2	Strong expression in the spiral ganglion and less in other restricted areas.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct epithelium	Present		2C3

Structure	Level	Pattern	Image	Note
TS24: semicircular canal	Present	Regionally restricted	2C4	Expression is in the cristae of the semicircular canals.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct epithelium	Present	Regionally restricted	2D	Expression is restricted to the presumptive pillar cells and to the more lateral Hensen/ Claudius cell domain.
TS26: tubotympanic recess	Present	Regionally restricted	2D	Expression is in the margin of the tympanic cavity.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct epithelium	Present	Regionally restricted	2D1	Expression is restricted to the presumptive pillar cells and to the more lateral Hensen/ Claudius cell domain.

Structure	Level	Pattern	Image	Note
TS26: crista ampullaris	Present	Regionally restricted	2E	Expression is in the cristae of the semicircular canals.

Structure	Level	Pattern	Image	Note
TS22: pinna surface epithelium	Strong		2J