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Gene Expression Data
Assay Details
Assay
Reference: J:97834 van den Akker E, et al., The common viral insertion site evi12 is located in the 5'-noncoding region of Gnn, a novel gene with enhanced expression in two subclasses of human acute myeloid leukemia. J Virol. 2005 May;79(9):5249-58
Assay type: Western blot
MGI Accession ID: MGI:3777262
Gene symbol: Nt5dc3
Gene name: 5'-nucleotidase domain containing 3
Antibody: Gnn antibody
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Proteins of various sizes ranging from ~25 - ~250 kDa could be detected in adult organs and embryos, whereas almost no Gnn protein was detected in bone marrow and spleen.
Results Image: 5
 Sample Information Bands Other Sample Information
Lane AgeStructure 250 kDa 150 kDa 65 kDa (a) Genetic BackgroundMutant Allele(s)Sex
NFS107 Cultured cells: no data stored
9.5 day embryo E9.5 (b) TS15: embryo Present Present Ambiguous Not Specified Not Specified
11.5 day embryo E11.5 (b) TS19: embryo Present Present Ambiguous Not Specified Not Specified
heart P adult TS28: heart Absent Present Strong Not Specified Not Specified
brain P adult TS28: brain Absent Present Ambiguous Not Specified Not Specified
kidney P adult TS28: metanephros Absent Present Strong Not Specified Not Specified
testis P adult TS28: testis Absent Present Present (c) Not Specified Male
thymus P adult TS28: thymus Absent Present Strong Not Specified Not Specified
muscle P adult TS28: musculature Absent Present Present (c) Not Specified Not Specified
spleen P adult TS28: spleen Absent Absent Ambiguous Not Specified Not Specified
stomach P adult TS28: stomach Absent Present Strong Not Specified Not Specified
large intestine P adult TS28: large intestine Absent Present Present (c) Not Specified Not Specified
lung P adult TS28: lung Absent Strong Ambiguous Not Specified Not Specified
liver P adult TS28: liver Absent Present Strong Not Specified Not Specified
small intestine P adult TS28: small intestine Absent Present Present (c) Not Specified Not Specified
bone marrow P adult TS28: bone marrow Absent Absent Ambiguous Not Specified Not Specified
Notes:
(a) Expression of Gnn proteins of ~65 kDa and ~55 kDa was evident in most organs with particularly high expression of the ~65-kDa protein in heart, kidney, thymus, stomach, and liver.
(b) Age of embryo at noon of plug day not specified in reference.
(c) 65kDa Present assigned by curator.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory